Reviewed, UniProtKB/Swiss-Prot A1BEN4 (NADE_CHLPD)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Cpha266_0810

Organism

Chlorobium phaeobacteroides (strain DSM 266) [Complete proteome] [HAMAP]

Taxonomic identifier

290317 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077545
Regions
	Nucleotide binding	36 – 43	8	ATP By similarity
Sites
	Active site	38	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1BEN4-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 48E7CAA35E0D3B4C	FASTA	277	31,086
	10 20 30 40 50 60 MNSLDLHLDY GIVEDILKTF LLNEIRKFGF RSVVLGLSGG IDSAVVCELA SRALGSDQVL 70 80 90 100 110 120 ALMMPYRSSS TDSIVHAQLL VEKLGIRAET CSITAAVDAF FEGVPEEDRL RRGNIMARTR 130 140 150 160 170 180 MVYLYDVSAR QNSLVVGTSN KTELLLGYGT LFGDMASAVN PVGDLYKTQI RGLARHLGIP 190 200 210 220 230 240 EQLITKTPSA DLWEGQSDEA DLGFSYDEVD HLLFMMLEKR MDKAAIIEQG VSEIFYDRVR 250 260 270 KMVVRNQYKR MMPVIAKISS RTPGIDFRYA RDWQEVK

References

[1]

"Complete sequence of Chlorobium phaeobacteroides DSM 266." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000492 Genomic DNA. Translation: ABL64861.1.
RefSeq	YP_911285.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1BEN4.
Genome annotation databases
GeneID	4570308.
GenomeReviews	Gene locus Cpha266_0810 in contig CP000492_GR.
KEGG	cph:Cpha266_0810.
NMPDR	fig|290317.7.peg.859.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CAINPIG.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CHLPD

Accession

Primary (citable) accession number: A1BEN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)