A1BDY0 (SYL_CHLPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	Cpha266_0551

Organism

Chlorobium phaeobacteroides (strain DSM 266) [Complete proteome] [HAMAP]

Taxonomic identifier

290317 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium › Chlorobium phaeobacteroides

Protein attributes

Sequence length	816 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: UniProtKB-HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 816	816	Leucine--tRNA ligase HAMAP-Rule MF_00049		PRO_1000009322
Regions
	Motif	40 – 51	12	"HIGH" region HAMAP-Rule MF_00049
	Motif	576 – 580	5	"KMSKS" region HAMAP-Rule MF_00049
Sites
	Binding site	579	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1BDY0 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 6770B1DAC50560F1	FASTA	816	93,335
	10 20 30 40 50 60 MRYDFSTIEK KWQSFWLKNN SFSSGESTDK PKYYVLDMFP YPSGSGLHVG HLEGYTATDI 70 80 90 100 110 120 VARYKRSCGY NVLHPMGWDA FGLPAEQFAI KTGTHPKNTT EKNISSFRET LQAMGFSYDW 130 140 150 160 170 180 SREINTTSPD YFRWTQWIFI KLYELGLAYV SEVDINWCEE LKTVLANEEV EEKVKEGYTV 190 200 210 220 230 240 IRKPLRQWVL KITAYAERLL DDLDELDWPE NVKQMQRNWI GRSEGMEIDF ELRCHNRKLR 250 260 270 280 290 300 VYTTRPDTLF GATFLVISPE HPLALKLATA QQLVAVTQYI KEAKLKSELE RTGLQKDKTG 310 320 330 340 350 360 IFTGSFAINP ATGDPLPVWI SDFVLTSYGT GAIMSVPAHD SRDWEFAKKF GLPIIEVIKS 370 380 390 400 410 420 PDGVDDAAFE GKESVSINSS NPEISIDGLH FSEAFQIMAH WLETKGLGER KVTYKLRDWI 430 440 450 460 470 480 FSRQRYWGEP IPVKHYEDGS IRTESNLPLQ LPEVEAYQPS ETGESPLATM HDWLYGADEF 490 500 510 520 530 540 GSFRRETNTM PQWAGSCWYY LRFIDPENNA QLIDPAKEKY WMNVDLYIGG AEHAVLHLLY 550 560 570 580 590 600 ARFWHKVLYD LGVVSSREPF KKLFNQGMIL GEDNEKMSKS RGNVIPADQV LKDYGADAVR 610 620 630 640 650 660 LYEMFLGPLE QVKPWNTNGI EGISRFLGKV WRLVYPDSEN PDQRDQAAVL DENPLPEILQ 670 680 690 700 710 720 RRMHKTIKKV TEDTDHLKFN TAISEMMVFV NELQKNGCRQ KSAVENLLLM LAPYAPHITA 730 740 750 760 770 780 ELWQAIGHTS PISAEPFPVF DPAIAEDPVV TIAVQVNGKL RGRFTAPAKS PEESLVDMAK 790 800 810 HVDTVIKFLE GQTIVKEIVI QDKLVNFAVK QSIPNH

References

[1]

"Complete sequence of Chlorobium phaeobacteroides DSM 266." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 266.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000492 Genomic DNA. Translation: ABL64607.1.
RefSeq	YP_911031.1. NC_008639.1.
3D structure databases
ProteinModelPortal	A1BDY0.
ModBase	Search...
Protein-protein interaction databases
STRING	290317.Cpha266_0551.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABL64607; ABL64607; Cpha266_0551.
GeneID	4569418.
KEGG	cph:Cpha266_0551.
PATRIC	21388885. VBIChlPha122104_0628.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000200748.
KO	K01869.
OMA	GIEHACM.
OrthoDB	EOG63Z74X.
ProtClustDB	PRK00390.
Enzyme and pathway databases
BioCyc	CPHA290317:GHX4-562-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF13603. tRNA-synt_1_2. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. SSF47323. 1 hit. SSF50677. SSF50677. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_CHLPD

Accession

Primary (citable) accession number: A1BDY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	November 13, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program