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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Chlorobium phaeobacteroides (strain DSM 266)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPHA290317:GHX4-562-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Cpha266_0551
OrganismiChlorobium phaeobacteroides (strain DSM 266)
Taxonomic identifieri290317 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
ProteomesiUP000008701: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 816816Leucine--tRNA ligasePRO_1000009322Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290317.Cpha266_0551.

Structurei

3D structure databases

ProteinModelPortaliA1BDY0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A1BDY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYDFSTIEK KWQSFWLKNN SFSSGESTDK PKYYVLDMFP YPSGSGLHVG
60 70 80 90 100
HLEGYTATDI VARYKRSCGY NVLHPMGWDA FGLPAEQFAI KTGTHPKNTT
110 120 130 140 150
EKNISSFRET LQAMGFSYDW SREINTTSPD YFRWTQWIFI KLYELGLAYV
160 170 180 190 200
SEVDINWCEE LKTVLANEEV EEKVKEGYTV IRKPLRQWVL KITAYAERLL
210 220 230 240 250
DDLDELDWPE NVKQMQRNWI GRSEGMEIDF ELRCHNRKLR VYTTRPDTLF
260 270 280 290 300
GATFLVISPE HPLALKLATA QQLVAVTQYI KEAKLKSELE RTGLQKDKTG
310 320 330 340 350
IFTGSFAINP ATGDPLPVWI SDFVLTSYGT GAIMSVPAHD SRDWEFAKKF
360 370 380 390 400
GLPIIEVIKS PDGVDDAAFE GKESVSINSS NPEISIDGLH FSEAFQIMAH
410 420 430 440 450
WLETKGLGER KVTYKLRDWI FSRQRYWGEP IPVKHYEDGS IRTESNLPLQ
460 470 480 490 500
LPEVEAYQPS ETGESPLATM HDWLYGADEF GSFRRETNTM PQWAGSCWYY
510 520 530 540 550
LRFIDPENNA QLIDPAKEKY WMNVDLYIGG AEHAVLHLLY ARFWHKVLYD
560 570 580 590 600
LGVVSSREPF KKLFNQGMIL GEDNEKMSKS RGNVIPADQV LKDYGADAVR
610 620 630 640 650
LYEMFLGPLE QVKPWNTNGI EGISRFLGKV WRLVYPDSEN PDQRDQAAVL
660 670 680 690 700
DENPLPEILQ RRMHKTIKKV TEDTDHLKFN TAISEMMVFV NELQKNGCRQ
710 720 730 740 750
KSAVENLLLM LAPYAPHITA ELWQAIGHTS PISAEPFPVF DPAIAEDPVV
760 770 780 790 800
TIAVQVNGKL RGRFTAPAKS PEESLVDMAK HVDTVIKFLE GQTIVKEIVI
810
QDKLVNFAVK QSIPNH
Length:816
Mass (Da):93,335
Last modified:January 23, 2007 - v1
Checksum:i6770B1DAC50560F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000492 Genomic DNA. Translation: ABL64607.1.
RefSeqiYP_911031.1. NC_008639.1.

Genome annotation databases

EnsemblBacteriaiABL64607; ABL64607; Cpha266_0551.
GeneIDi4569418.
KEGGicph:Cpha266_0551.
PATRICi21388885. VBIChlPha122104_0628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000492 Genomic DNA. Translation: ABL64607.1.
RefSeqiYP_911031.1. NC_008639.1.

3D structure databases

ProteinModelPortaliA1BDY0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290317.Cpha266_0551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL64607; ABL64607; Cpha266_0551.
GeneIDi4569418.
KEGGicph:Cpha266_0551.
PATRICi21388885. VBIChlPha122104_0628.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCPHA290317:GHX4-562-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Chlorobium phaeobacteroides DSM 266."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 266.

Entry informationi

Entry nameiSYL_CHLPD
AccessioniPrimary (citable) accession number: A1BDY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.