A1BDJ5 (ISPH_CHLPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Cpha266_0414

Organism

Chlorobium phaeobacteroides (strain DSM 266) [Complete proteome] [HAMAP]

Taxonomic identifier

290317 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021101

Sequences

	Sequence		Length	Mass (Da)
	A1BDJ5 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 2F58AEF09DCC8910	FASTA	328	36,521
	10 20 30 40 50 60 MKINLDRTSS GFCIGVQGTI HVAEEKLNNP DKLFSLGDVV HNEAEVNRLE HLGLTTIDES 70 80 90 100 110 120 GFHELKNAQL LIRAHGEPPS TYRIAEKNNL VITDTTCPVV SRLQRTARLL HELGYQIIIY 130 140 150 160 170 180 GKPSHPEVIG INGHCKNSAV IIKHPDLSDP SETGQIDLKR KTALISQTTM DVPGFYELKA 190 200 210 220 230 240 NIRNLFAADS EEIRNENDHL WMAIRDIDLT ASLTGIRDMP RYVYKDTICR QVSSRNQMLH 250 260 270 280 290 300 DFAIANSCVI FVAGKKSSNG QVLFGICKAA NPCTYFIEDI DEIDDAWLTG SDGRTVESVG 310 320 ICGATSTPMW LLEKVAAHLQ RRYNHDPA

References

[1]

"Complete sequence of Chlorobium phaeobacteroides DSM 266." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 266.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000492 Genomic DNA. Translation: ABL64472.1.
RefSeq	YP_910896.1. NC_008639.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1BDJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4570968.
GenomeReviews	Gene locus Cpha266_0414 in contig CP000492_GR.
KEGG	cph:Cpha266_0414.
NMPDR	fig|290317.7.peg.425.
PATRIC	21388559. VBIChlPha122104_0466.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	HNQGKRY.
ProtClustDB	PRK01045.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPH_CHLPD

Accession

Primary (citable) accession number: A1BDJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program