A1BCQ2 (A1BCQ2_CHLPD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Cpha266_0109

Organism

Chlorobium phaeobacteroides (strain DSM 266) [Complete proteome] [HAMAP]

Taxonomic identifier

290317 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	799 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A1BCQ2 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 125C6AAF4A2F0E4D	FASTA	799	89,767
	10 20 30 40 50 60 MALKSPAASA RNRTLIVVES PSKAKTINKY LGDKYTVFAS VGHIKDLPKK EIGLDFDHHY 70 80 90 100 110 120 EPRYEVIAGK EKVVRQLKKL ADESNEILIA TDPDREGEAI AWHIANEVEF AHKPVFRVLF 130 140 150 160 170 180 NEITKTAIIA AIQQPRQIDY RLVRSQQTRQ GLDKIVGYKI SPFLWNVVLR GLSAGRVQSV 190 200 210 220 230 240 ALRLICEREA EINGFQIQEY WSIAADFKTD LNETFRTKLI KIKGDKPELS NQKKAETAAA 250 260 270 280 290 300 EISERLFAVK EITTRVQQRK PPFPFTTSLL QQAASNQLGF GSQKTMRIAQ QLYEGIELGD 310 320 330 340 350 360 EGATGLITYM RTDSIRIGTE ATAEARNYIT AQFGKEYVGY GGAAKAGKNA QDAHEAIRPT 370 380 390 400 410 420 SLLRKPESVK HFLSSDQFRL YELIWRRLLT AMMAPAKIEQ TRVDVEDREK EFTFRANGSR 430 440 450 460 470 480 VLFAGFMRVY DDQQELDYEA QSSTRDDVEK EQTVQLPEKL KEQDKLGLAG LDSKQSFTRP 490 500 510 520 530 540 PARYSEATLV KDLDNYGIGR PSTYASIFST LQDRRYVELQ KKKIIPTELG KDVSLILVAN 550 560 570 580 590 600 FPELFNVGFT AHMEDELDKV ASGDDEYEAV LEKFYRPLET ALSLRKSDPI IPQNSATETC 610 620 630 640 650 660 DKCGKGQMTV KWTASGKFLG CSRYPSCKNI KPLSSSKAKP KDTGIQCPSC KEGHMLLRDG 670 680 690 700 710 720 RLGPFLACSS YPKCNTLLNL GKQRQVEPMK IPPLLTTLVC PKCGAPMNLR SGKRGLWLGC 730 740 750 760 770 780 SKFPKCRGRL AWGTLDEEVQ RHWETLMTEH QKAHPTVALT MIDGRPVPLT LSVDDIITRA 790 EEAGLIAAVP EEQQELTAE

References

[1]

"Complete sequence of Chlorobium phaeobacteroides DSM 266." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 266.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000492 Genomic DNA. Translation: ABL64179.1.
RefSeq	YP_910603.1. NC_008639.1.
3D structure databases
ProteinModelPortal	A1BCQ2.
ModBase	Search...
Protein-protein interaction databases
STRING	A1BCQ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4569463.
GenomeReviews	Gene locus Cpha266_0109 in contig CP000492_GR.
KEGG	cph:Cpha266_0109.
NMPDR	fig|290317.7.peg.114.
PATRIC	21387876. VBIChlPha122104_0134.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	QELDETC.
PhylomeDB	A1BCQ2.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1BCQ2_CHLPD

Accession

Primary (citable) accession number: A1BCQ2

Entry history

Integrated into UniProtKB/TrEMBL:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)