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Protein

Glutamate 5-kinase

Gene

proB

Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17ATPUniRule annotation1
Binding sitei56SubstrateUniRule annotation1
Binding sitei143SubstrateUniRule annotation1
Binding sitei155Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi175 – 176ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPDEN318586:G1GW1-4713-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Pden_4055
OrganismiParacoccus denitrificans (strain Pd 1222)
Taxonomic identifieri318586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
Proteomesi
  • UP000000361 Componenti: Chromosome 2

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000810841 – 370Glutamate 5-kinaseAdd BLAST370

Proteomic databases

PRIDEiA1B9C7

Interactioni

Protein-protein interaction databases

STRINGi318586.Pden_4055

Structurei

3D structure databases

ProteinModelPortaliA1B9C7
SMRiA1B9C7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 357PUAUniRule annotationAdd BLAST78

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

A1B9C7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVTPKLGR AQRLVVKIGS ALLVNGAGLR AEWLRALCDD VAQARARGTD
60 70 80 90 100
VVLVSSGAIA LGRQVLGLPA GPLRVEQSQA AAAVGQIKLA RAYEEALAPH
110 120 130 140 150
GVKTAQLLVT LDDTTDRRRY LNSRATMQTL LGLGVVPIVN ENDTVATDEI
160 170 180 190 200
RFGDNDRLAA QIAVTCGADQ LLLLSDVDGL YTANPKTDPT ARHLPVVGQI
210 220 230 240 250
TPEIEAMGGD PISGVSKGGM KTKLLAARTA VAGGCAMAIA EGSVLNPLSA
260 270 280 290 300
VAQGARVTWF LPDTDPQAAR KRWIAAMKPK GEITVDAGAA LALGQGKSLL
310 320 330 340 350
PAGVTAVSGR FGRGDPVVVL DGKGGRLASG LVRYSSTEAR AIAGHRSDEI
360 370
EGILGYPGRA ALIHRDDMVV
Length:370
Mass (Da):38,317
Last modified:January 23, 2007 - v1
Checksum:i3F6B0AB5283FD149
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000490 Genomic DNA Translation: ABL72121.1
RefSeqiWP_011750289.1, NC_008687.1

Genome annotation databases

EnsemblBacteriaiABL72121; ABL72121; Pden_4055
KEGGipde:Pden_4055

Similar proteinsi

Entry informationi

Entry nameiPROB_PARDP
AccessioniPrimary (citable) accession number: A1B9C7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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