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A1B656

- BIOB2_PARDP

UniProt

A1B656 - BIOB2_PARDP

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Protein
Biotin synthase 2
Gene
bioB2, Pden_2916
Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi53 – 531Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi93 – 931Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi124 – 1241Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi184 – 1841Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi256 – 2561Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPDEN318586:GCVQ-2958-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase 2 (EC:2.8.1.6)
Gene namesi
Name:bioB2
Ordered Locus Names:Pden_2916
OrganismiParacoccus denitrificans (strain Pd 1222)
Taxonomic identifieri318586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
ProteomesiUP000000361: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Biotin synthase 2UniRule annotation
PRO_0000381516Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi318586.Pden_2916.

Structurei

3D structure databases

ProteinModelPortaliA1B656.
SMRiA1B656. Positions 10-309.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1B656-1 [UniParc]FASTAAdd to Basket

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MPQQSRSAAE IYHQPLMDLL FQAQTVHRAH FDPNVVQCSK LLSIKTGGCP    50
EDCAYCSQSA RNGSELSASK LMEVQRVLAE ARRAKEAGAT RYCMGAAWRS 100
PKERDMPAVL AMIRGVKAMG METCMTLGML DADQALRLKD AGLDYYNHNI 150
DTSERYYSEI ITTRTFQDRI ETLERVQAAG INVCAGGIVG MGETAEDRIS 200
MLETLAGLEV PPQSVPINML MPMAGTPLAD VPRLDAIEMV RTIATARILM 250
PASYVRLSAG RSEMSDEMQA MCFFAGANSI FVGDTLLTAG NPDEDKDALL 300
FAKLGLRAEV PEASQEGCAA 320
Length:320
Mass (Da):34,800
Last modified:January 23, 2007 - v1
Checksum:i48800D3D05F55D23
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000490 Genomic DNA. Translation: ABL71000.1.
RefSeqiWP_011749190.1. NC_008687.1.
YP_916696.1. NC_008687.1.

Genome annotation databases

EnsemblBacteriaiABL71000; ABL71000; Pden_2916.
GeneIDi4581483.
KEGGipde:Pden_2916.
PATRICi22856986. VBIParDen97112_2857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000490 Genomic DNA. Translation: ABL71000.1 .
RefSeqi WP_011749190.1. NC_008687.1.
YP_916696.1. NC_008687.1.

3D structure databases

ProteinModelPortali A1B656.
SMRi A1B656. Positions 10-309.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 318586.Pden_2916.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABL71000 ; ABL71000 ; Pden_2916 .
GeneIDi 4581483.
KEGGi pde:Pden_2916.
PATRICi 22856986. VBIParDen97112_2857.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PDEN318586:GCVQ-2958-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pd 1222.

Entry informationi

Entry nameiBIOB2_PARDP
AccessioniPrimary (citable) accession number: A1B656
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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