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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei624 – 6241ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPDEN318586:GCVQ-2806-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Pden_2765
OrganismiParacoccus denitrificans (strain Pd 1222)
Taxonomic identifieri318586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
ProteomesiUP000000361 Componenti: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 844844Leucine--tRNA ligasePRO_0000334787Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi318586.Pden_2765.

Structurei

3D structure databases

ProteinModelPortaliA1B5Q5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 4911"HIGH" regionAdd
BLAST
Motifi621 – 6255"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1B5Q5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYDPAISEP RWQEAWEQAD TFRAVRDERP KYYVLEMFPY PSGRIHMGHV
60 70 80 90 100
RNYTMGDVVA RFKRAQGFSV LHPMGWDAFG MPAENAAMEQ GGHPRDWTYG
110 120 130 140 150
NIATMRGQLK PLGLSIDWSR EFATCDDAYV AQQQALFLDM LEAGLITRKS
160 170 180 190 200
AQVNWDPVDM TVLANEQVID GKGWRSGATV ERKELTQWFF KISDYSDELL
210 220 230 240 250
SALDGLEGWP EKVRLMQANW IGKSRGLQFR FHTVDLPDFP TIEVYTTRPD
260 270 280 290 300
TLMGASFVAL SPDHPLVKAL AASDPKVAAF VEECRRIGTT EEAIETAPKM
310 320 330 340 350
GFDTGLTVRH PLDADWRLPI WIANFVLMDY GTGAIFGSPA HDERDHEFAT
360 370 380 390 400
KYGLPIRATF GERGMDLEQA DALVAKAPFV PLKSETVTYV RGFAGAADQT
410 420 430 440 450
GEAAVDAAIR HAEVAGYGEG VTKFRLRDWG ISRQRYWGCP IPVVHCDACG
460 470 480 490 500
TVPEAKANLP VLLPQDVSFE VPGNPLNRHP TWAATTCPKC GGPARRETDT
510 520 530 540 550
MDTFVDSSWY YARFTSPHAA TPTDRPETDY WMNVDQYIGG IEHAILHLLY
560 570 580 590 600
SRFFARAMVK TGHLPESAKE PFDALFTQGM VTHEIYMTRD ERGRPVYHLP
610 620 630 640 650
EDVVDGKLAD GTPVEVIPSA KMSKSKKNVV DPVNIVEGFG ADTARWFMLS
660 670 680 690 700
DSPPERDVEW TAAGAEAANR FLARVWRLAD EIPEDGADPD LTRAAHRAID
710 720 730 740 750
EVTRAIEGFA FNKAVAKIYE LANAIAKSGA GGESRREALR IMAQLMAPMV
760 770 780 790 800
PHLAEEIWMK AGGQGMVVDA TWPKADPALL VSDSVVLPIQ INGKRRAEIE
810 820 830 840
VPKDMPKDQI EAIVLADETV RRFMEGQAPK KLIVVPGRIV NVVV
Length:844
Mass (Da):93,879
Last modified:January 22, 2007 - v1
Checksum:i1DE1B82D42F62E75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000489 Genomic DNA. Translation: ABL70849.1.
RefSeqiYP_916545.1. NC_008686.1.

Genome annotation databases

EnsemblBacteriaiABL70849; ABL70849; Pden_2765.
KEGGipde:Pden_2765.
PATRICi22856677. VBIParDen97112_2704.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000489 Genomic DNA. Translation: ABL70849.1.
RefSeqiYP_916545.1. NC_008686.1.

3D structure databases

ProteinModelPortaliA1B5Q5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi318586.Pden_2765.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL70849; ABL70849; Pden_2765.
KEGGipde:Pden_2765.
PATRICi22856677. VBIParDen97112_2704.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPDEN318586:GCVQ-2806-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pd 1222.

Entry informationi

Entry nameiSYL_PARDP
AccessioniPrimary (citable) accession number: A1B5Q5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 19, 2008
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.