A1B4Z0 (KATG_PARDP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Pden_2497

Organism

Paracoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]

Taxonomic identifier

318586 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Catalase-peroxidase HAMAP MF_01961		PRO_0000354853
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1B4Z0 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 52AFEBBE3A3857B5	FASTA	725	79,623
	10 20 30 40 50 60 MDGNDIRSTG KCPVMHGGNT AMGSSVTAWW PNALNLDILH QHDSKTNPLG QDFNYSEELK 70 80 90 100 110 120 KLDVEALKAD LRALMTDSQD WWPADWGSYV GMFARTAWHM AGSYRTSDGR GGANTGNQRF 130 140 150 160 170 180 APLNSWPDNV NTDKGRRLLW PIKKKYGNKI SWADLIVLAG TVAYEAAGLK TYGFAFGRED 190 200 210 220 230 240 IWHPEKDTYW GEEKEWLAPS DSRYGDVTKA ETLANPLAAV QMGLIYVNPE GVNGKSDPQA 250 260 270 280 290 300 TAYQMRETFA RMGMNDEETV ALTAGGHTVG KCHGNGNVTD LSPDPEAAGP EFQGLGWMNT 310 320 330 340 350 360 KGRGIGRNTM VSGLEGAWTT HPTQWDNGFF EMLFKHEWTL THSPAGASQW EPITIAEEDK 370 380 390 400 410 420 PADVEDPSIR HMPMMTDADM ALKVDPIYRE ISLRFMNDFE AFSDAFARAW FKLTHRDMGP 430 440 450 460 470 480 KARYLGPDVP AEDLVWQDPI PAGRSDYDVA AVKARIAASG LSASDLVATA WDSARTYRGS 490 500 510 520 530 540 DHRGGANGAR IRLAPQKDWE GNEPQRLARV LSVLEPIAAE TGASLADVIV LGGNLGVEQA 550 560 570 580 590 600 AKAAGFDIEV PFAPGRGDST AEQTDAASFD VLEPLADGFR NWQKQDYVVS PEEMLLDRAQ 610 620 630 640 650 660 LMGLTAPEMT TLIGGLRVIG TNHGGTKHGV FTQRKGALTN DFFVTLTDMA NNWVPVGNNL 670 680 690 700 710 720 YEIRDRKTGA TRYTATRVDL VFGSNSILRA YAEVYAQDDN AGKFVRDFVA AWTKVMNADR FDLAA

References

[1]

"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pd 1222.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000489 Genomic DNA. Translation: ABL70584.1.
RefSeq	YP_916280.1. NC_008686.1.
3D structure databases
ProteinModelPortal	A1B4Z0.
SMR	A1B4Z0. Positions 25-723.
ModBase	Search...
Protein-protein interaction databases
STRING	A1B4Z0.
Protein family/group databases
PeroxiBase	3626. PdeCP01_PD1222.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4580135.
GenomeReviews	Gene locus Pden_2497 in contig CP000489_GR.
KEGG	pde:Pden_2497.
PATRIC	22856117. VBIParDen97112_2423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	A1B4Z0.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. False negative. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_PARDP

Accession

Primary (citable) accession number: A1B4Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program