NADH-quinone oxidoreductase subunit B - Paracoccus denitrificans (strain Pd 1222)

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Reviewed, UniProtKB/Swiss-Prot A1B497 (NUOB_PARDP)

Last modified February 9, 2010. Version 28. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit B
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit B
    NDH-1 subunit B
    NADH-quinone oxidoreductase subunit 6
      Short name=NQO6
    NADH dehydrogenase I, subunit 6
    NDH-1, subunit 6

Gene names

Name:	nuoB
Synonyms:	nqo6
Ordered Locus Names:	Pden_2249

Organism

Paracoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]

Taxonomic identifier

318586 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

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Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01356
Catalytic activity	NADH + quinone = NAD⁺ + quinol. HAMAP MF_01356
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_01356
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers By similarity. NADH-quinone oxidoreductase forms a supercomplex with ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) and cytochrome c oxidase (complex IV), which stabilizes the NADH-quinone oxidoreductase complex. HAMAP MF_01356
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side Probable HAMAP MF_01356.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

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Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 175

175

NADH-quinone oxidoreductase subunit B

PRO_0000358441

Sites

Metal binding

Iron-sulfur (4Fe-4S) Potential

Metal binding

Iron-sulfur (4Fe-4S) Potential

Metal binding

119

Iron-sulfur (4Fe-4S) Potential

Metal binding

149

Iron-sulfur (4Fe-4S) Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

A1B497-1 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 8233131871D66E8B
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FASTA

175

19,505

        10         20         30         40         50         60 
MMTGLNTAGA DRDLATAELN RELQDKGFLL TTTEDIINWA RNGSLHWMTF GLACCAVEMM 

        70         80         90        100        110        120 
QTSMPRYDLE RFGTAPRASP RQSDLMIVAG TLTNKMAPAL RKVYDQMPEP RYVISMGSCA 

       130        140        150        160        170 
NGGGYYHYSY SVVRGCDRIV PVDIYVPGCP PTAEALLYGI LQLQRRIRRT GTLVR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Assembly of respiratory complexes I, III, and IV into NADH oxidase supercomplex stabilizes complex I in Paracoccus denitrificans." Stroh A., Anderka O., Pfeiffer K., Yagi T., Finel M., Ludwig B., Schagger H. J. Biol. Chem. 279:5000-5007(2004) [PubMed: 14610094] [Abstract] Cited for: IDENTIFICATION IN NADH OXIDASE SUPERCOMPLEX, SUBUNIT, SUBCELLULAR LOCATION.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000489 Genomic DNA. Translation: ABL70341.1.
RefSeq	YP_916037.1.
3D structure databases
SMR	A1B497. Positions 24-172.
ModBase	Search...
Protein-protein interaction databases
STRING	A1B497.
Genome annotation databases
GeneID	4579303.
GenomeReviews	Gene locus Pden_2249 in contig CP000489_GR.
KEGG	pde:Pden_2249.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0377.
HOGENOM	HBG553221.
OMA	EMMATNA.
PhylomeDB	A1B497.
Family and domain databases
HAMAP	MF_01356. NDH1_NuoB. [Tree]
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UbQ_OxRdtase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

NUOB_PARDP

Accession

Primary (citable) accession number: A1B497

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents