A1B495 (NUOD_PARDP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit D
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I, subunit 4
NADH dehydrogenase I, subunit D
NADH-quinone oxidoreductase subunit 4
Short name=NQO4
NDH-1, subunit 4
NDH-1, subunit D

Gene names

Name:	nuoD
Synonyms:	nqo4
Ordered Locus Names:	Pden_2247

Organism

Paracoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]

Taxonomic identifier

318586 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01358
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01358
Subunit structure	NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers By similarity. NADH-quinone oxidoreductase forms a supercomplex with ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) and cytochrome c oxidase (complex IV), which stabilizes the NADH-quinone oxidoreductase complex. Ref.2
Subcellular location	Cell inner membrane; Peripheral membrane protein Probable Ref.2.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	NADH-quinone oxidoreductase subunit D HAMAP MF_01358		PRO_0000357882

Sequences

	Sequence		Length	Mass (Da)
	A1B495 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 9D22F7ABA02151FE	FASTA	412	46,724
	10 20 30 40 50 60 MDGDIRKNSY DDGSMDALTG EQSIRNFNIN FGPQHPAAHG VLRMVLELDG EIVERADPHI 70 80 90 100 110 120 GLLHRGTEKL MESRTYLQNL PYLDRLDYVA PMNQEHAWCL AIERLTGTVI PRRASLIRVL 130 140 150 160 170 180 YSEIGRILNH LMGVTTGAMD VGALTPPLWG FEAREELMIF YERACGARLH AAYFRPGGVH 190 200 210 220 230 240 QDLPPDLLDD IEEWCERFPK LVDDLDTLLT ENRIFKQRLV DIGIVTEADA LDWGYTGVMV 250 260 270 280 290 300 RGSGLAWDLR RSQPYECYDE FDFQIPVGRN GDCYDRYLCR MAEMRESCKI MQQAVQKLRA 310 320 330 340 350 360 EPAGDVLARG KLTPPRRAEM KRDMESLIHH FKLYTEGFKV PAGEVYAAVE APKGEFGVYL 370 380 390 400 410 VADGTNKPWR AKLRAPGFAH LQSIDWMSRG HMLADVPAII ATLDIVFGEV DR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pd 1222.
[2]	"Assembly of respiratory complexes I, III, and IV into NADH oxidase supercomplex stabilizes complex I in Paracoccus denitrificans." Stroh A., Anderka O., Pfeiffer K., Yagi T., Finel M., Ludwig B., Schagger H. J. Biol. Chem. 279:5000-5007(2004) [PubMed: 14610094] [Abstract] Cited for: IDENTIFICATION IN NADH OXIDASE SUPERCOMPLEX, SUBUNIT, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000489 Genomic DNA. Translation: ABL70339.1.
RefSeq	YP_916035.1. NC_008686.1.
3D structure databases
ProteinModelPortal	A1B495.
ModBase	Search...
Protein-protein interaction databases
STRING	A1B495.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4579301.
GenomeReviews	Gene locus Pden_2247 in contig CP000489_GR.
KEGG	pde:Pden_2247.
PATRIC	22855623. VBIParDen97112_2180.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0649.
HOGENOM	HBG459705.
OMA	IFYERAC.
PhylomeDB	A1B495.
ProtClustDB	PRK06075.
Family and domain databases
HAMAP	MF_01358. NDH1_NuoD. [Tree]
InterPro	IPR010219. NADH_DH_1_suD. IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR022885. NDH1_su_D/H. [Graphical view]
KO	K00333.
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOD_PARDP

Accession

Primary (citable) accession number: A1B495

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program