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Protein

NADH-quinone oxidoreductase subunit N

Gene

nuoN

Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.1 Publication

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit N (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit 14
NADH dehydrogenase I subunit N
NADH-quinone oxidoreductase subunit 14
Short name:
NQO14
NDH-1 subunit 14
NDH-1 subunit N
Gene namesi
Name:nuoN
Synonyms:nqo141 Publication
Ordered Locus Names:Pden_2231
OrganismiParacoccus denitrificans (strain Pd 1222)
Taxonomic identifieri318586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
Proteomesi
  • UP000000361 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Transmembranei76 – 96HelicalSequence analysisAdd BLAST21
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
Transmembranei235 – 255HelicalSequence analysisAdd BLAST21
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
Transmembranei301 – 321HelicalSequence analysisAdd BLAST21
Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
Transmembranei369 – 389HelicalSequence analysisAdd BLAST21
Transmembranei402 – 422HelicalSequence analysisAdd BLAST21
Transmembranei446 – 466HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003911971 – 499NADH-quinone oxidoreductase subunit NAdd BLAST499

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of at least 14 different subunits, Nqo1 to Nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8, Nqo10 to Nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers (By similarity). NADH-quinone oxidoreductase forms a supercomplex with ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) and cytochrome c oxidase (complex IV), which stabilizes the NADH-quinone oxidoreductase complex (PubMed:14610094).By similarity1 Publication

Protein-protein interaction databases

STRINGi318586.Pden_2231.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CNR. Bacteria.
COG1007. LUCA.
HOGENOMiHOG000100795.
KOiK00343.
OMAiDQMALFF.
OrthoDBiPOG091H0B2L.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1. 1 hit.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.

Sequencei

Sequence statusi: Complete.

A1B479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLDFSTIL PEVVLAGYAL AALMAGAYLG KDRLARTLLW VTVAAFLVVA
60 70 80 90 100
AMVGLGNHVD GAAFHGMFID DGFSRFAKVV TLVAAAGVLA MSADYMQRRN
110 120 130 140 150
MLRFEFPIIV ALAVLGMMFM VSAGDLLTLY MGLELQSLAL YVVAAMRRDS
160 170 180 190 200
VRSSEAGLKY FVLGSLSSGL LLYGASLVYG FAGTTGFEGI ISTIEAGHLS
210 220 230 240 250
LGVLFGLVFM LVGLSFKVSA VPFHMWTPDV YEGSPTPVTA FFATAPKVAA
260 270 280 290 300
MALIARLVFD AFGHVIGDWS QIVAALAVMS MFLGSIAGIG QTNIKRLMAY
310 320 330 340 350
SSIAHMGFAL VGLAAGTAIG VQNMLLYMTI YAVMNIGTFA FILSMERDGV
360 370 380 390 400
PVTDLAALNR FAWTDPVKAL AMLVLMFSLA GVPPTLGFFA KFGVLTAAVD
410 420 430 440 450
AGMGWLAVLG VIASVIGAFY YLRIVYYMYF GGESEGMTSR MGAVQYLALM
460 470 480 490
VPALAMLVGA ISMFGVDSAA GRAAETLVGP VAAIEQPAEA AQAEPVQGE
Length:499
Mass (Da):52,535
Last modified:January 23, 2007 - v1
Checksum:iF116A0CEA09ED72A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000489 Genomic DNA. Translation: ABL70323.1.
RefSeqiWP_011748518.1. NC_008686.1.

Genome annotation databases

EnsemblBacteriaiABL70323; ABL70323; Pden_2231.
KEGGipde:Pden_2231.
PATRICi22855587. VBIParDen97112_2162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000489 Genomic DNA. Translation: ABL70323.1.
RefSeqiWP_011748518.1. NC_008686.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi318586.Pden_2231.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL70323; ABL70323; Pden_2231.
KEGGipde:Pden_2231.
PATRICi22855587. VBIParDen97112_2162.

Phylogenomic databases

eggNOGiENOG4105CNR. Bacteria.
COG1007. LUCA.
HOGENOMiHOG000100795.
KOiK00343.
OMAiDQMALFF.
OrthoDBiPOG091H0B2L.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1. 1 hit.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNUON_PARDP
AccessioniPrimary (citable) accession number: A1B479
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.