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A1B479 (NUON_PARDP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit N

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit 14
NADH dehydrogenase I subunit N
NADH-quinone oxidoreductase subunit 14
Short name=NQO14
NDH-1 subunit 14
NDH-1 subunit N
Gene names
Name:nuoN
Synonyms:nqo14
Ordered Locus Names:Pden_2231
OrganismParacoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]
Taxonomic identifier318586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_00445

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_00445

Subunit structure

NDH-1 is composed of at least 14 different subunits, nqo1 to nqo14. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8, nqo10 to nqo14) embedded in the inner membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers By similarity. NADH-quinone oxidoreductase forms a supercomplex with ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) and cytochrome c oxidase (complex IV), which stabilizes the NADH-quinone oxidoreductase complex. Ref.2

Subcellular location

Cell inner membrane; Multi-pass membrane protein Probable Ref.2.

Sequence similarities

Belongs to the complex I subunit 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499NADH-quinone oxidoreductase subunit N HAMAP-Rule MF_00445
PRO_0000391197

Regions

Transmembrane9 – 2921Helical; Potential
Transmembrane37 – 5721Helical; Potential
Transmembrane76 – 9621Helical; Potential
Transmembrane104 – 12421Helical; Potential
Transmembrane126 – 14621Helical; Potential
Transmembrane161 – 18121Helical; Potential
Transmembrane196 – 21621Helical; Potential
Transmembrane235 – 25521Helical; Potential
Transmembrane269 – 28921Helical; Potential
Transmembrane301 – 32121Helical; Potential
Transmembrane324 – 34421Helical; Potential
Transmembrane369 – 38921Helical; Potential
Transmembrane402 – 42221Helical; Potential
Transmembrane446 – 46621Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
A1B479 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: F116A0CEA09ED72A

FASTA49952,535
        10         20         30         40         50         60 
MTSLDFSTIL PEVVLAGYAL AALMAGAYLG KDRLARTLLW VTVAAFLVVA AMVGLGNHVD 

        70         80         90        100        110        120 
GAAFHGMFID DGFSRFAKVV TLVAAAGVLA MSADYMQRRN MLRFEFPIIV ALAVLGMMFM 

       130        140        150        160        170        180 
VSAGDLLTLY MGLELQSLAL YVVAAMRRDS VRSSEAGLKY FVLGSLSSGL LLYGASLVYG 

       190        200        210        220        230        240 
FAGTTGFEGI ISTIEAGHLS LGVLFGLVFM LVGLSFKVSA VPFHMWTPDV YEGSPTPVTA 

       250        260        270        280        290        300 
FFATAPKVAA MALIARLVFD AFGHVIGDWS QIVAALAVMS MFLGSIAGIG QTNIKRLMAY 

       310        320        330        340        350        360 
SSIAHMGFAL VGLAAGTAIG VQNMLLYMTI YAVMNIGTFA FILSMERDGV PVTDLAALNR 

       370        380        390        400        410        420 
FAWTDPVKAL AMLVLMFSLA GVPPTLGFFA KFGVLTAAVD AGMGWLAVLG VIASVIGAFY 

       430        440        450        460        470        480 
YLRIVYYMYF GGESEGMTSR MGAVQYLALM VPALAMLVGA ISMFGVDSAA GRAAETLVGP 

       490 
VAAIEQPAEA AQAEPVQGE 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pd 1222.
[2]"Assembly of respiratory complexes I, III, and IV into NADH oxidase supercomplex stabilizes complex I in Paracoccus denitrificans."
Stroh A., Anderka O., Pfeiffer K., Yagi T., Finel M., Ludwig B., Schagger H.
J. Biol. Chem. 279:5000-5007(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN NADH OXIDASE SUPERCOMPLEX, SUBUNIT, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000489 Genomic DNA. Translation: ABL70323.1.
RefSeqYP_916019.1. NC_008686.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318586.Pden_2231.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL70323; ABL70323; Pden_2231.
GeneID4579285.
KEGGpde:Pden_2231.
PATRIC22855587. VBIParDen97112_2162.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1007.
HOGENOMHOG000100795.
KOK00343.
OMAPIGEFAA.
OrthoDBEOG64JFNZ.
ProtClustDBPRK05777.

Enzyme and pathway databases

BioCycPDEN318586:GCVQ-2271-MONOMER.

Family and domain databases

HAMAPMF_00445. NDH1_NuoN_1.
InterProIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01770. NDH_I_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNUON_PARDP
AccessionPrimary (citable) accession number: A1B479
Entry history
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families