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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei93S-adenosyl-L-methionineUniRule annotation1
Binding sitei120S-adenosyl-L-methionineUniRule annotation1
Active sitei142By similarity1
Binding sitei142S-adenosyl-L-methionineUniRule annotation1
Binding sitei146SubstrateUniRule annotation1
Binding sitei178SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPDEN318586:G1GW1-2249-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Pden_2227
OrganismiParacoccus denitrificans (strain Pd 1222)
Taxonomic identifieri318586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
Proteomesi
  • UP000000361 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881941 – 233tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST233

Proteomic databases

PRIDEiA1B475

Interactioni

Protein-protein interaction databases

STRINGi318586.Pden_2227

Structurei

3D structure databases

ProteinModelPortaliA1B475
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 214Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073969
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

A1B475-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFDPNPPR RNFYGRRHGK TLRQSQKGYL SEDLGSLRPR GITVQENPER
60 70 80 90 100
KPVAPSTIFG DDRPVWLEVG FGGGEHMVHM AARYPEIGII GCEPFINGVA
110 120 130 140 150
MLLGKIRAAG VENVSVHPGD ARDLMDVLPD ASIDKAFLNY PDPWPKARHH
160 170 180 190 200
RRRFVTPEHL IPLARVMKPG AEFRVATDIP DYVRQTLEEV PQAGFDLVAE
210 220 230
GPQAWEDWPS TRYEQKALRE GRVPHYVTFR RRS
Length:233
Mass (Da):26,471
Last modified:January 23, 2007 - v1
Checksum:iC3FC3A378E52A6A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000489 Genomic DNA Translation: ABL70319.1
RefSeqiWP_011748514.1, NC_008686.1

Genome annotation databases

EnsemblBacteriaiABL70319; ABL70319; Pden_2227
KEGGipde:Pden_2227

Similar proteinsi

Entry informationi

Entry nameiTRMB_PARDP
AccessioniPrimary (citable) accession number: A1B475
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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