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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei188SubstrateUniRule annotation1
Active sitei189Proton donor/acceptorUniRule annotation1
Active sitei319UniRule annotation1
Binding sitei320SubstrateUniRule annotation1
Sitei332Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:Pden_1908Imported
OrganismiParacoccus denitrificans (strain Pd 1222)Imported
Taxonomic identifieri318586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
Proteomesi
  • UP000000361 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi318586.Pden_1908.

Structurei

3D structure databases

ProteinModelPortaliA1B3A9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 343Lyase_1InterPro annotationAdd BLAST331
Domaini409 – 462FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 101Substrate bindingUniRule annotation3
Regioni130 – 133Substrate binding (B site)UniRule annotation4
Regioni140 – 142Substrate bindingUniRule annotation3
Regioni325 – 327Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A1B3A9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTTRTETD SFGPLEVPAD KYWGAQTQRS IQNFPIGWER QPVPIIRALG
60 70 80 90 100
AIKQAAAEVN MAAGKLDPAI GKAMVQAASE VVAGRFDDNF PLVVWQTGSG
110 120 130 140 150
TQSNMNANEV ISNRAIEILG GEMGSKKPVH PNDHVNMGQS SNDTFPTAMH
160 170 180 190 200
VAIAMQARDE LLPGLEKLHK ALVAKSEEFK DIIKIGRTHT QDATPLTLGQ
210 220 230 240 250
EFGGYAHQVA KGIERVKLAL GDIYELAQGG TAVGTGLNTR KGWDTAIAAK
260 270 280 290 300
IAEITGLPFV TAPNKFEALA AHDAMVMFSG ALKTVAASLF KIANDMRLLG
310 320 330 340 350
SGPRSGLGEL ILPENEPGSS IMPGKVNPTQ AEALTMVCAH VMGNDAAVGF
360 370 380 390 400
AGSQGHFELN VYNPMMSYNV LQSMQLLGDA AGSFTDNMVV GTQANIPRID
410 420 430 440 450
KLMKESLMLV TALAPTIGYD NATKVAKTAH KNGTTLREEA IALGFVDGET
460
FDRIVRPEQM IGPED
Length:465
Mass (Da):49,580
Last modified:January 23, 2007 - v1
Checksum:i23A3D03D7E79D369
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000489 Genomic DNA. Translation: ABL70003.1.
RefSeqiWP_011748200.1. NC_008686.1.

Genome annotation databases

EnsemblBacteriaiABL70003; ABL70003; Pden_1908.
KEGGipde:Pden_1908.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiA1B3A9_PARDP
AccessioniPrimary (citable) accession number: A1B3A9
Entry historyiIntegrated into UniProtKB/TrEMBL: January 23, 2007
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported