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A1B1Z0 (BIOB1_PARDP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase 1

EC=2.8.1.6
Gene names
Name:bioB1
Ordered Locus Names:Pden_1433
OrganismParacoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]
Taxonomic identifier318586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Biotin synthase 1 HAMAP-Rule MF_01694
PRO_0000381515

Sites

Metal binding521Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1271Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1871Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2601Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A1B1Z0 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: C6A9662808D64651

FASTA32435,023
        10         20         30         40         50         60 
MIRTDWTMAE AWAIHALPFA DLMHRAQTLH RAHFDPNAIE TASLLSIKTG GCPEDCGYCS 

        70         80         90        100        110        120 
QSAHHDTGVK ATKLMGTEEV LAAARRAKAS GAQRFCMGAA WRSPKDRDMD KLCDMVRGVA 

       130        140        150        160        170        180 
ELGLETCMTL GMLSPEQVAR LKAAGLDFYN HNIDTSPEYY AQIASTRTME NRLDTVEQVR 

       190        200        210        220        230        240 
KGGIKVCCGG ILGMGEAEED RIAMLVTLAT LPAHPDSVPV NLWNEIEGVP VQARAQAVDP 

       250        260        270        280        290        300 
FALVRIVALA RILMPASVVR LSAGRTGMSD ELQALCFLAG ANSIFVGDQL LTTGNPAAWK 

       310        320 
DQDLLSRLGM HIAPAQARPR VAAE 

« Hide

References

[1]"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pd 1222.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000489 Genomic DNA. Translation: ABL69534.1.
RefSeqYP_915230.1. NC_008686.1.

3D structure databases

ProteinModelPortalA1B1Z0.
SMRA1B1Z0. Positions 3-312.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318586.Pden_1433.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL69534; ABL69534; Pden_1433.
GeneID4580488.
KEGGpde:Pden_1433.
PATRIC22854007. VBIParDen97112_1394.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAPMESFEV.
OrthoDBEOG622PMP.
ProtClustDBCLSK2310309.

Enzyme and pathway databases

BioCycPDEN318586:GCVQ-1449-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB1_PARDP
AccessionPrimary (citable) accession number: A1B1Z0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways