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A1B1T3

- LIPA_PARDP

UniProt

A1B1T3 - LIPA_PARDP

Protein

Lipoyl synthase

Gene

lipA

Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi80 – 801Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi84 – 841Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPDEN318586:GCVQ-1392-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Pden_1376
    OrganismiParacoccus denitrificans (strain Pd 1222)
    Taxonomic identifieri318586 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
    ProteomesiUP000000361: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Lipoyl synthasePRO_0000325283Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi318586.Pden_1376.

    Structurei

    3D structure databases

    ProteinModelPortaliA1B1T3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiFERIATN.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A1B1T3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADMPPVLRH PEKAHRPDQP QPKKPDWIRV KAPTSQGYKD TRDILRNNRL    50
    STVCEEAGCP NVGECWSQGH ATMMIMGEIC TRGCSFCNVM TGKPNALDVF 100
    EPGRVAHAVQ KLGLKHVVIT SVDRDDLDDG GAEHFAQTIR AIRHRSPAST 150
    IEVLTPDFLK SKPGALEAVV EARPDVFNHN LETVPGLYPT VRPGARYFHS 200
    LRLLQQVKEL DPGMFTKSGI MVGLGEDRQG ILQVMDDMRA ADVDFITIGQ 250
    YLQPTPKHHR VDRFVTPEEF KGYEKAAYGK GFLMVSATPL TRSSYHAGDD 300
    FAQLRAARLA KLGRA 315
    Length:315
    Mass (Da):34,980
    Last modified:January 23, 2007 - v1
    Checksum:i3DDEAF58BBF4D607
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000489 Genomic DNA. Translation: ABL69477.1.
    RefSeqiYP_915173.1. NC_008686.1.

    Genome annotation databases

    EnsemblBacteriaiABL69477; ABL69477; Pden_1376.
    GeneIDi4579807.
    KEGGipde:Pden_1376.
    PATRICi22853899. VBIParDen97112_1340.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000489 Genomic DNA. Translation: ABL69477.1 .
    RefSeqi YP_915173.1. NC_008686.1.

    3D structure databases

    ProteinModelPortali A1B1T3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 318586.Pden_1376.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABL69477 ; ABL69477 ; Pden_1376 .
    GeneIDi 4579807.
    KEGGi pde:Pden_1376.
    PATRICi 22853899. VBIParDen97112_1340.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi FERIATN.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci PDEN318586:GCVQ-1392-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pd 1222.

    Entry informationi

    Entry nameiLIPA_PARDP
    AccessioniPrimary (citable) accession number: A1B1T3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3