A1B0F1 (SYE1_PARDP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Pden_0884

Organism

Paracoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]

Taxonomic identifier

318586 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367729
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	239 – 243	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1B0F1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: F686B873732310FA	FASTA	441	48,902
	10 20 30 40 50 60 MTTTRFAPSP TGHIHVGNLR TALMNYLIAR KAGGTFILRL DDTDRERSKQ EYADGIQRDL 70 80 90 100 110 120 EWLGLTWDRI ERQSDRLDRY AEAAEGLRAA GRLYEVFETP TELDLKRKKQ LNMGKPPVYD 130 140 150 160 170 180 RAGLKLSAED KDRLRAEGRA GYWRFLLDQE RIEWADGILG DISIDAASVS DPVLIRADGQ 190 200 210 220 230 240 VLYTFASSVD DAEMGVTHIV RGADHVTNTA TQIQIIRALG SEPPAFAHHS LLTGAQGEEL 250 260 270 280 290 300 SKRLGTLSIR DLRESGVAPE ALLSLMARLG SSQPVELKMS LDELAEGFDL GQFGASPTKF 310 320 330 340 350 360 DAEDLWPLTR EANQSRPLAE VRDRIAALGV PDELVERFWR VASQNITKLD DLAGWWQIFS 370 380 390 400 410 420 TGAEPQIDPE DADFIAEAMK LLPPPPYTDA TWGEFTNAVK QATGRKGKGL FMPLRKALTG 430 440 QAHGPDMSEV MPLLQVVRAR G

References

[1]

"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pd 1222.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000489 Genomic DNA. Translation: ABL68995.1.
RefSeq	YP_914691.1. NC_008686.1.
3D structure databases
ProteinModelPortal	A1B0F1.
ModBase	Search...
Protein-protein interaction databases
STRING	A1B0F1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4579280.
GenomeReviews	Gene locus Pden_0884 in contig CP000489_GR.
KEGG	pde:Pden_0884.
PATRIC	22852930. VBIParDen97112_0862.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DQERIEW.
ProtClustDB	PRK12558.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_PARDP

Accession

Primary (citable) accession number: A1B0F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program