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A1AX29 (DDL_RUTMC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Rmag_0759
OrganismRuthia magnifica subsp. Calyptogena magnifica [Complete proteome] [HAMAP]
Taxonomic identifier413404 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbionts

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 293293D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341171

Regions

Domain98 – 291194ATP-grasp
Nucleotide binding124 – 17754ATP By similarity

Sites

Metal binding2451Magnesium or manganese 1 By similarity
Metal binding2581Magnesium or manganese 1 By similarity
Metal binding2581Magnesium or manganese 2 By similarity
Metal binding2601Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1AX29 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 662304CAEC52EA68

FASTA29332,800
        10         20         30         40         50         60 
MIAVLMGGNS AERAVSLKSG EAVYQALINQ NINCFEFDWH GYNLSELWQQ EFDQAFIVLH 

        70         80         90        100        110        120 
GRGGEDGYIQ KQLENRCIRY TGSDSNASHN GIDKARTKII WKQHNLTLAP SIVASIHKPI 

       130        140        150        160        170        180 
EPIDFPLPWM VKPTLEGSSI GISKVDSQIQ LNNALMLAWQ YNSHALIEQW IEGDEYTVAI 

       190        200        210        220        230        240 
LGDKALPVVK IITDQGFYDY ESKYHSNKIQ YLCPCGLSSS QEQVLQVIAL KAFFAINAKG 

       250        260        270        280        290 
WGRVDFIINQ HNKPYLLEIN TVPGMTSHSL VPMAAKAMGM SFNKLVVAII NEI 

« Hide

References

[1]"The Calyptogena magnifica chemoautotrophic symbiont genome."
Newton I.L.G., Woyke T., Auchtung T.A., Dilly G.F., Dutton R.J., Fisher M.C., Fontanez K.M., Lau E., Stewart F.J., Richardson P.M., Barry K.W., Saunders E., Detter J.C., Wu D., Eisen J.A., Cavanaugh C.M.
Science 315:998-1000(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000488 Genomic DNA. Translation: ABL02486.1.
RefSeqYP_903957.1. NC_008610.1.

3D structure databases

ProteinModelPortalA1AX29.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING413404.Rmag_0759.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL02486; ABL02486; Rmag_0759.
GeneID4555677.
KEGGrma:Rmag_0759.
PATRIC32001249. VBICanRut45856_0837.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAEDPARES.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycCRUT413404:GHM7-785-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_RUTMC
AccessionPrimary (citable) accession number: A1AX29
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways