A1AWV4 (CPDA_RUTMC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA
Short name=3',5'-cyclic AMP phosphodiesterase
Short name=cAMP phosphodiesterase
EC=3.1.4.17

Gene names

Name:	cpdA
Ordered Locus Names:	Rmag_0669

Organism

Ruthia magnifica subsp. Calyptogena magnifica [Complete proteome] [HAMAP]

Taxonomic identifier

413404 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › unclassified Gammaproteobacteria › sulfur-oxidizing symbionts › Candidatus Ruthia magnifica

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes By similarity. HAMAP-Rule MF_00905
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. HAMAP-Rule MF_00905
Cofactor	Binds 2 metal cations per subunit By similarity.
Sequence similarities	Belongs to the cAMP phosphodiesterase class-III family.

Ontologies

Keywords
Ligand	Metal-binding Nucleotide-binding cAMP
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA HAMAP-Rule MF_00905		PRO_0000413378
Regions
	Nucleotide binding	79 – 80	2	cAMP By similarity
Sites
	Metal binding	11	1	Metal cation 1 By similarity
	Metal binding	13	1	Metal cation 1 By similarity
	Metal binding	49	1	Metal cation 1 By similarity
	Metal binding	49	1	Metal cation 2 By similarity
	Metal binding	79	1	Metal cation 2 By similarity
	Metal binding	145	1	Metal cation 2 By similarity
	Metal binding	184	1	Metal cation 2 By similarity
	Metal binding	186	1	Metal cation 1 By similarity
	Binding site	13	1	cAMP By similarity
	Binding site	49	1	cAMP By similarity
	Binding site	186	1	cAMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1AWV4 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 75601DAEC907D5D5	FASTA	234	26,547
	10 20 30 40 50 60 MMSHSFVQIS DCHIDNVEYA MGVNTHVNLK KIINKIINID TDALLISGDL THNGTITSYK 70 80 90 100 110 120 TLQQILFPVQ IKLLVISGNH DIDNNLNTIF SKNLFSQFTL RKWEIISTNS VQTSKTSGFL 130 140 150 160 170 180 TKNELKKLNF NLTQSIAKYI LIVLHHPIVP MNSTWDDSLS LENPKALFNV LDKYPKIQAI 190 200 210 220 230 LFGHAHQAAE FSRLGVKIIS CPSTALQFNN ETRIGFNHYT LYDNGQLTID TQWI

References

[1]

"The Calyptogena magnifica chemoautotrophic symbiont genome." Newton I.L.G., Woyke T., Auchtung T.A., Dilly G.F., Dutton R.J., Fisher M.C., Fontanez K.M., Lau E., Stewart F.J., Richardson P.M., Barry K.W., Saunders E., Detter J.C., Wu D., Eisen J.A., Cavanaugh C.M. Science 315:998-1000(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000488 Genomic DNA. Translation: ABL02411.1.
RefSeq	YP_903882.1. NC_008610.1.
3D structure databases
ProteinModelPortal	A1AWV4.
ModBase	Search...
Protein-protein interaction databases
STRING	413404.Rmag_0669.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABL02411; ABL02411; Rmag_0669.
GeneID	4554602.
KEGG	rma:Rmag_0669.
PATRIC	32001053. VBICanRut45856_0739.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1409.
HOGENOM	HOG000137387.
KO	K03651.
OMA	IPVFWIP.
ProtClustDB	CLSK850669.
Enzyme and pathway databases
BioCyc	CRUT413404:GHM7-710-MONOMER.
Family and domain databases
HAMAP	MF_00905. cAMP_phophodiest_CpdA.
InterPro	IPR026575. cAMP_Pdiest_CpdA. IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CPDA_RUTMC

Accession

Primary (citable) accession number: A1AWV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program