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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Ruthia magnifica subsp. Calyptogena magnifica
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi96ZincUniRule annotation1
Metal bindingi98ZincUniRule annotation1
Metal bindingi123ZincUniRule annotation1
Metal bindingi125ZincUniRule annotation1
Binding sitei233ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCRUT413404:G1G7M-484-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Rmag_0474
OrganismiRuthia magnifica subsp. Calyptogena magnifica
Taxonomic identifieri413404 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbiontsCandidatus Ruthia
Proteomesi
  • UP000002587 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003309941 – 454Glutamate--tRNA ligaseAdd BLAST454

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi413404.Rmag_0474

Structurei

3D structure databases

ProteinModelPortaliA1AWC3
SMRiA1AWC3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 17"HIGH" regionUniRule annotationAdd BLAST11
Motifi230 – 234"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A1AWC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSRFAPSPT GCLHIGGART ALFAWVWAKK QHGKFVLRIE DTDLERSNQA
60 70 80 90 100
SVDAILQGMN WLGLDYDEGP FYQTDRFNRY KQVVRQLLDE KKAYYCECSK
110 120 130 140 150
ERLQILHEDL IKQGKKVRYD GCCRDKNLND GVVRFNNPED GLVIFNDVIK
160 170 180 190 200
GQISINNKEL DDLIIVRSDG TPTYNLTVVV DDHDMQIDCV IRGDDHINNT
210 220 230 240 250
PKQINLYQAL GWHLPEFAHL PMILSSDGAR LSKRHDAVSI MTYRDAGFLP
260 270 280 290 300
EALLNYLARL GWSYGDQEIF SMDEIVKLFE LKSINKAPAS FNQDKLLWLN
310 320 330 340 350
QKIIKNSSVE NLLNNLTWHL QNQAITITNA PNIEAVVQYL QNRCKTLVDM
360 370 380 390 400
AGEVKMFYQD FDTFDEKLAK RYLKDKTPLK HLFAKLEALR IWKANNIKQA
410 420 430 440 450
VKEVCFELNI SFGKVGQPFR LALSGNGNAG SIDIVAELVG KNKALSRLKM

AIDS
Length:454
Mass (Da):51,820
Last modified:January 23, 2007 - v1
Checksum:iC7FE21E05A226755
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000488 Genomic DNA Translation: ABL02230.1
RefSeqiWP_011737855.1, NC_008610.1

Genome annotation databases

EnsemblBacteriaiABL02230; ABL02230; Rmag_0474
KEGGirma:Rmag_0474

Similar proteinsi

Entry informationi

Entry nameiSYE_RUTMC
AccessioniPrimary (citable) accession number: A1AWC3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health