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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Ruthia magnifica subsp. Calyptogena magnifica
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi96 – 961ZincUniRule annotation
Metal bindingi98 – 981ZincUniRule annotation
Metal bindingi123 – 1231ZincUniRule annotation
Metal bindingi125 – 1251ZincUniRule annotation
Binding sitei233 – 2331ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCRUT413404:GHM7-494-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Rmag_0474
OrganismiRuthia magnifica subsp. Calyptogena magnifica
Taxonomic identifieri413404 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbiontsCandidatus Ruthia
ProteomesiUP000002587: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Glutamate--tRNA ligasePRO_0000330994Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi413404.Rmag_0474.

Structurei

3D structure databases

ProteinModelPortaliA1AWC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi7 – 1711"HIGH" regionAdd
BLAST
Motifi230 – 2345"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiVCEIDDM.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1AWC3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSRFAPSPT GCLHIGGART ALFAWVWAKK QHGKFVLRIE DTDLERSNQA
60 70 80 90 100
SVDAILQGMN WLGLDYDEGP FYQTDRFNRY KQVVRQLLDE KKAYYCECSK
110 120 130 140 150
ERLQILHEDL IKQGKKVRYD GCCRDKNLND GVVRFNNPED GLVIFNDVIK
160 170 180 190 200
GQISINNKEL DDLIIVRSDG TPTYNLTVVV DDHDMQIDCV IRGDDHINNT
210 220 230 240 250
PKQINLYQAL GWHLPEFAHL PMILSSDGAR LSKRHDAVSI MTYRDAGFLP
260 270 280 290 300
EALLNYLARL GWSYGDQEIF SMDEIVKLFE LKSINKAPAS FNQDKLLWLN
310 320 330 340 350
QKIIKNSSVE NLLNNLTWHL QNQAITITNA PNIEAVVQYL QNRCKTLVDM
360 370 380 390 400
AGEVKMFYQD FDTFDEKLAK RYLKDKTPLK HLFAKLEALR IWKANNIKQA
410 420 430 440 450
VKEVCFELNI SFGKVGQPFR LALSGNGNAG SIDIVAELVG KNKALSRLKM

AIDS
Length:454
Mass (Da):51,820
Last modified:January 23, 2007 - v1
Checksum:iC7FE21E05A226755
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000488 Genomic DNA. Translation: ABL02230.1.
RefSeqiYP_903701.1. NC_008610.1.

Genome annotation databases

EnsemblBacteriaiABL02230; ABL02230; Rmag_0474.
GeneIDi4554799.
KEGGirma:Rmag_0474.
PATRICi32000621. VBICanRut45856_0529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000488 Genomic DNA. Translation: ABL02230.1.
RefSeqiYP_903701.1. NC_008610.1.

3D structure databases

ProteinModelPortaliA1AWC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi413404.Rmag_0474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL02230; ABL02230; Rmag_0474.
GeneIDi4554799.
KEGGirma:Rmag_0474.
PATRICi32000621. VBICanRut45856_0529.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiVCEIDDM.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciCRUT413404:GHM7-494-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYE_RUTMC
AccessioniPrimary (citable) accession number: A1AWC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.