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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ruthia magnifica subsp. Calyptogena magnifica
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei552Aminoacyl-adenylateUniRule annotation1
Binding sitei596ATPUniRule annotation1
Metal bindingi885ZincUniRule annotation1
Metal bindingi888ZincUniRule annotation1
Metal bindingi905ZincUniRule annotation1
Metal bindingi908ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCRUT413404:G1G7M-356-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Rmag_0340
OrganismiRuthia magnifica subsp. Calyptogena magnifica
Taxonomic identifieri413404 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbiontsCandidatus Ruthia
Proteomesi
  • UP000002587 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221131 – 922Isoleucine--tRNA ligaseAdd BLAST922

Proteomic databases

PRIDEiA1AW09

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi413404.Rmag_0340

Structurei

3D structure databases

ProteinModelPortaliA1AW09
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi593 – 597"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A1AW09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKSSLNL PATQFPMKAN LANREGGFLK KWQNDGLYDQ IRQQNQGKPK
60 70 80 90 100
FVLHDGPPYA NGDIHIGHTV NKVLKDMIVK SKSLSGFDAP YVPGWDCHGL
110 120 130 140 150
PIEFNVEKKY GKVGVKIDAS TFRSKCRKYA DQQVLKQKQD FQRLGILSDW
160 170 180 190 200
DNPYLTKDFQ YESDIVRALG RIVKNGHVSK GYKPVHWCTE CGSALAETEV
210 220 230 240 250
EYKDNQSEAI DVKFRIIDDS VFNMNKPVSV VIWTTTPWTL PANEAVALHP
260 270 280 290 300
ELNYVLVDIG DEYLLLAQAL VETSISRYGI EATIGEQTFS GSELEGLKIK
310 320 330 340 350
HPFYDKQVPI ILGEYVTIDA GTGAVHTAPA HGQEDFIVGL KYNLPVECLV
360 370 380 390 400
DIKGVFFKET ELLGGQFIFK ANDSVIEILK QANTLVKHES LMHSYPHCWR
410 420 430 440 450
HKTPVIFRAT PQWFISMQKN GLIDTVNREI SKVQWMPDWG KKRIELMMDS
460 470 480 490 500
RPDWCISRQR FWGVPITLFV HKQTGELHPN TQALFVSIAN RIEKEGIEAW
510 520 530 540 550
FKSDAQDFIG NDANDYDKTT DILDVWFDSG VSHFTVLKAR KELSNVADLY
560 570 580 590 600
LEGADQHRGW FQLSLISSVA INGKAPYKNV LTHGFVVDKN GKKMSKSLGN
610 620 630 640 650
VMSPQKIVNN LGADVLRLWI ASTDYTSEMT VGDEILKRSA DSYRRIRNTM
660 670 680 690 700
RFMLANMQAF NPVEHLLDNK QMLDLDKWIV AKTANLQTQI IKAYEQYNFH
710 720 730 740 750
HVVQLILNFC SNDLGGFYLD VIKDRQYTTQ ANSLARRSAQ SALHHITEAM
760 770 780 790 800
VRWLAPILSF TAEEIWQNMP SEKNTSIFLA SWYQDLTFGY ENKAIDITRE
810 820 830 840 850
ISPFIRKQME GMRGEKIIGS SLESEIDIYC ETDIFSTLSQ LDDELRFIFI
860 870 880 890 900
TSYIRIHSLN EKNDDCIKAI EGVFIKVSKS RHEKCVRCWH HREDVGNNAK
910 920
HPELCGRCVE NVDGKGEIRK FA
Length:922
Mass (Da):105,262
Last modified:January 23, 2007 - v1
Checksum:iCF5316BC7F9E8F8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000488 Genomic DNA Translation: ABL02116.1
RefSeqiWP_011737741.1, NC_008610.1

Genome annotation databases

EnsemblBacteriaiABL02116; ABL02116; Rmag_0340
KEGGirma:Rmag_0340

Similar proteinsi

Entry informationi

Entry nameiSYI_RUTMC
AccessioniPrimary (citable) accession number: A1AW09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health