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A1AW09

- SYI_RUTMC

UniProt

A1AW09 - SYI_RUTMC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ruthia magnifica subsp. Calyptogena magnifica
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation
    Metal bindingi885 – 8851ZincUniRule annotation
    Metal bindingi888 – 8881ZincUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCRUT413404:GHM7-353-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Rmag_0340
    OrganismiRuthia magnifica subsp. Calyptogena magnifica
    Taxonomic identifieri413404 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbionts
    ProteomesiUP000002587: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 922922Isoleucine--tRNA ligasePRO_1000022113Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi413404.Rmag_0340.

    Structurei

    3D structure databases

    ProteinModelPortaliA1AW09.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKKRIELM.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1AW09-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKSSLNL PATQFPMKAN LANREGGFLK KWQNDGLYDQ IRQQNQGKPK    50
    FVLHDGPPYA NGDIHIGHTV NKVLKDMIVK SKSLSGFDAP YVPGWDCHGL 100
    PIEFNVEKKY GKVGVKIDAS TFRSKCRKYA DQQVLKQKQD FQRLGILSDW 150
    DNPYLTKDFQ YESDIVRALG RIVKNGHVSK GYKPVHWCTE CGSALAETEV 200
    EYKDNQSEAI DVKFRIIDDS VFNMNKPVSV VIWTTTPWTL PANEAVALHP 250
    ELNYVLVDIG DEYLLLAQAL VETSISRYGI EATIGEQTFS GSELEGLKIK 300
    HPFYDKQVPI ILGEYVTIDA GTGAVHTAPA HGQEDFIVGL KYNLPVECLV 350
    DIKGVFFKET ELLGGQFIFK ANDSVIEILK QANTLVKHES LMHSYPHCWR 400
    HKTPVIFRAT PQWFISMQKN GLIDTVNREI SKVQWMPDWG KKRIELMMDS 450
    RPDWCISRQR FWGVPITLFV HKQTGELHPN TQALFVSIAN RIEKEGIEAW 500
    FKSDAQDFIG NDANDYDKTT DILDVWFDSG VSHFTVLKAR KELSNVADLY 550
    LEGADQHRGW FQLSLISSVA INGKAPYKNV LTHGFVVDKN GKKMSKSLGN 600
    VMSPQKIVNN LGADVLRLWI ASTDYTSEMT VGDEILKRSA DSYRRIRNTM 650
    RFMLANMQAF NPVEHLLDNK QMLDLDKWIV AKTANLQTQI IKAYEQYNFH 700
    HVVQLILNFC SNDLGGFYLD VIKDRQYTTQ ANSLARRSAQ SALHHITEAM 750
    VRWLAPILSF TAEEIWQNMP SEKNTSIFLA SWYQDLTFGY ENKAIDITRE 800
    ISPFIRKQME GMRGEKIIGS SLESEIDIYC ETDIFSTLSQ LDDELRFIFI 850
    TSYIRIHSLN EKNDDCIKAI EGVFIKVSKS RHEKCVRCWH HREDVGNNAK 900
    HPELCGRCVE NVDGKGEIRK FA 922
    Length:922
    Mass (Da):105,262
    Last modified:January 23, 2007 - v1
    Checksum:iCF5316BC7F9E8F8A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000488 Genomic DNA. Translation: ABL02116.1.
    RefSeqiYP_903587.1. NC_008610.1.

    Genome annotation databases

    EnsemblBacteriaiABL02116; ABL02116; Rmag_0340.
    GeneIDi4554628.
    KEGGirma:Rmag_0340.
    PATRICi32000323. VBICanRut45856_0386.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000488 Genomic DNA. Translation: ABL02116.1 .
    RefSeqi YP_903587.1. NC_008610.1.

    3D structure databases

    ProteinModelPortali A1AW09.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 413404.Rmag_0340.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABL02116 ; ABL02116 ; Rmag_0340 .
    GeneIDi 4554628.
    KEGGi rma:Rmag_0340.
    PATRICi 32000323. VBICanRut45856_0386.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KKRIELM.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CRUT413404:GHM7-353-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiSYI_RUTMC
    AccessioniPrimary (citable) accession number: A1AW09
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3