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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ruthia magnifica subsp. Calyptogena magnifica
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi885 – 8851ZincUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCRUT413404:GHM7-353-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Rmag_0340
OrganismiRuthia magnifica subsp. Calyptogena magnifica
Taxonomic identifieri413404 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbiontsCandidatus Ruthia
ProteomesiUP000002587 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 922922Isoleucine--tRNA ligasePRO_1000022113Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi413404.Rmag_0340.

Structurei

3D structure databases

ProteinModelPortaliA1AW09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1AW09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKSSLNL PATQFPMKAN LANREGGFLK KWQNDGLYDQ IRQQNQGKPK
60 70 80 90 100
FVLHDGPPYA NGDIHIGHTV NKVLKDMIVK SKSLSGFDAP YVPGWDCHGL
110 120 130 140 150
PIEFNVEKKY GKVGVKIDAS TFRSKCRKYA DQQVLKQKQD FQRLGILSDW
160 170 180 190 200
DNPYLTKDFQ YESDIVRALG RIVKNGHVSK GYKPVHWCTE CGSALAETEV
210 220 230 240 250
EYKDNQSEAI DVKFRIIDDS VFNMNKPVSV VIWTTTPWTL PANEAVALHP
260 270 280 290 300
ELNYVLVDIG DEYLLLAQAL VETSISRYGI EATIGEQTFS GSELEGLKIK
310 320 330 340 350
HPFYDKQVPI ILGEYVTIDA GTGAVHTAPA HGQEDFIVGL KYNLPVECLV
360 370 380 390 400
DIKGVFFKET ELLGGQFIFK ANDSVIEILK QANTLVKHES LMHSYPHCWR
410 420 430 440 450
HKTPVIFRAT PQWFISMQKN GLIDTVNREI SKVQWMPDWG KKRIELMMDS
460 470 480 490 500
RPDWCISRQR FWGVPITLFV HKQTGELHPN TQALFVSIAN RIEKEGIEAW
510 520 530 540 550
FKSDAQDFIG NDANDYDKTT DILDVWFDSG VSHFTVLKAR KELSNVADLY
560 570 580 590 600
LEGADQHRGW FQLSLISSVA INGKAPYKNV LTHGFVVDKN GKKMSKSLGN
610 620 630 640 650
VMSPQKIVNN LGADVLRLWI ASTDYTSEMT VGDEILKRSA DSYRRIRNTM
660 670 680 690 700
RFMLANMQAF NPVEHLLDNK QMLDLDKWIV AKTANLQTQI IKAYEQYNFH
710 720 730 740 750
HVVQLILNFC SNDLGGFYLD VIKDRQYTTQ ANSLARRSAQ SALHHITEAM
760 770 780 790 800
VRWLAPILSF TAEEIWQNMP SEKNTSIFLA SWYQDLTFGY ENKAIDITRE
810 820 830 840 850
ISPFIRKQME GMRGEKIIGS SLESEIDIYC ETDIFSTLSQ LDDELRFIFI
860 870 880 890 900
TSYIRIHSLN EKNDDCIKAI EGVFIKVSKS RHEKCVRCWH HREDVGNNAK
910 920
HPELCGRCVE NVDGKGEIRK FA
Length:922
Mass (Da):105,262
Last modified:January 23, 2007 - v1
Checksum:iCF5316BC7F9E8F8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000488 Genomic DNA. Translation: ABL02116.1.
RefSeqiYP_903587.1. NC_008610.1.

Genome annotation databases

EnsemblBacteriaiABL02116; ABL02116; Rmag_0340.
KEGGirma:Rmag_0340.
PATRICi32000323. VBICanRut45856_0386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000488 Genomic DNA. Translation: ABL02116.1.
RefSeqiYP_903587.1. NC_008610.1.

3D structure databases

ProteinModelPortaliA1AW09.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi413404.Rmag_0340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL02116; ABL02116; Rmag_0340.
KEGGirma:Rmag_0340.
PATRICi32000323. VBICanRut45856_0386.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCRUT413404:GHM7-353-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYI_RUTMC
AccessioniPrimary (citable) accession number: A1AW09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.