Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfp

Organism
Ruthia magnifica subsp. Calyptogena magnifica
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Non-allosteric.UniRule annotation

Pathway: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111Diphosphate; via amide nitrogenUniRule annotation
Sitei113 – 1131Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation
Sitei139 – 1391Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation
Active sitei142 – 1421Proton acceptorUniRule annotation
Binding sitei242 – 2421SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciCRUT413404:GHM7-98-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfpUniRule annotation
Ordered Locus Names:Rmag_0094Imported
OrganismiRuthia magnifica subsp. Calyptogena magnificaImported
Taxonomic identifieri413404 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbiontsCandidatus Ruthia
ProteomesiUP000002587 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi413404.Rmag_0094.

Structurei

3D structure databases

ProteinModelPortaliA1AVD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 1423Substrate bindingUniRule annotation
Regioni188 – 1903Substrate bindingUniRule annotation
Regioni292 – 2954Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade "B2" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000007357.
KOiK00850.
OMAiCGVIETA.
OrthoDBiEOG6PP9HS.

Family and domain databases

HAMAPiMF_01978. Phosphofructokinase_II_B2.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011404. PPi-PFK_XF0274.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036483. PFK_XF0274. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

A1AVD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNAFYAQSG GVTAVINASA CGVIETVRKH PDKIGTIYAG QNGIIGALTE
60 70 80 90 100
NLIDTSKESN ADIKALKHTP SGGFGSCRYK MKSLETNKAE YERLIEVFKE
110 120 130 140 150
YDVGYFFYNG GGDSADTCLK VSQLSKSMGY PIQAIHVPKT VDNDLPITDN
160 170 180 190 200
CPGFGSVAKY IAVSIMEASF DVASMAATST KIFVLEVMGR HAGWIAAAGG
210 220 230 240 250
LVDNSIPVVI LFPEVDFDEK KFLAKVNKNV KEFNYCTIVV SEGAKWPDGC
260 270 280 290 300
FLAEQGTHDD FGNAQLGGAA PVVANLIKDT LGYKYHWAVA DYLQRAARHL
310 320 330 340 350
ASKSDVEQAY ALGVAAVNMA LEGKNSVMPT IIRTSNNPYT WEIGSGELKD
360 370 380 390 400
VANVEKMMPM NYISNDGFGI TDACREYLQP LIEGEDYPPY KNGLPDYVVM
410
KKIMTDKKLP TFEI
Length:414
Mass (Da):45,071
Last modified:January 23, 2007 - v1
Checksum:i7792544F1A9FF904
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000488 Genomic DNA. Translation: ABL01893.1.
RefSeqiWP_011737519.1. NC_008610.1.
YP_903364.1. NC_008610.1.

Genome annotation databases

EnsemblBacteriaiABL01893; ABL01893; Rmag_0094.
KEGGirma:Rmag_0094.
PATRICi31999753. VBICanRut45856_0109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000488 Genomic DNA. Translation: ABL01893.1.
RefSeqiWP_011737519.1. NC_008610.1.
YP_903364.1. NC_008610.1.

3D structure databases

ProteinModelPortaliA1AVD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi413404.Rmag_0094.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL01893; ABL01893; Rmag_0094.
KEGGirma:Rmag_0094.
PATRICi31999753. VBICanRut45856_0109.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000007357.
KOiK00850.
OMAiCGVIETA.
OrthoDBiEOG6PP9HS.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciCRUT413404:GHM7-98-MONOMER.

Family and domain databases

HAMAPiMF_01978. Phosphofructokinase_II_B2.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011404. PPi-PFK_XF0274.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036483. PFK_XF0274. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CmImported.

Entry informationi

Entry nameiA1AVD6_RUTMC
AccessioniPrimary (citable) accession number: A1AVD6
Entry historyi
Integrated into UniProtKB/TrEMBL: January 23, 2007
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.