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A1AV52 (SYL_RUTMC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Rmag_0005
OrganismRuthia magnifica subsp. Calyptogena magnifica [Complete proteome] [HAMAP]
Taxonomic identifier413404 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriasulfur-oxidizing symbionts

Protein attributes

Sequence length816 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 816816Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009420

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif574 – 5785"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5771ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1AV52 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: B464A5B928F544BF

FASTA81693,408
        10         20         30         40         50         60 
MNSEYNAQKI EAQAQKYWQE NKSFEVSEDT SKEKYYCLSM FPYPSGSLHM GHVRNYSIGD 

        70         80         90        100        110        120 
VISRYQKMQG KNVMQPIGWD GFGLPAENAA LENKVSPAKW TYENIDYMRD QLSKLGFGYD 

       130        140        150        160        170        180 
WSREIATCHQ KYYRWEQWLF IKLFEKDLVY KKNAIVNWDP VDQTVLANEQ VIDGRGWRSN 

       190        200        210        220        230        240 
ALIEKKKISQ WFMRITNYAD ELIYGLDKLD GWPDAVKTMQ KNWIGKSVGL EVDFPRHNAD 

       250        260        270        280        290        300 
SIKVYTTRPD TLMGVTYLVI SSEHPIALEA GKNNPQVQAF IDECKTIQIT KETMKTIDKK 

       310        320        330        340        350        360 
GIDLGVKCIH PITSDDVPIW IANFVLIGYG TGAVMSVPAH DKRDYEFAKK YGIAIKKVIN 

       370        380        390        400        410        420 
ENISIDKNAM TDKGVLFNSG EFNGLNFDQA FNEIAKTLTD KNLGRKKTNY RLQDWGISRQ 

       430        440        450        460        470        480 
RYWGCPIPII NCQNCGIVVV PEADLPVVLP EVMSFDSVGS LIKKMPEFYQ TTCPKCGKMA 

       490        500        510        520        530        540 
QQETDTLDTF FESSWYFARY TCKDNNDKML DKRANYWLAN GGVDQYIGGI EHAILHLLYA 

       550        560        570        580        590        600 
RFFNKLLRDE GFIKNDEPFK NLLTQGMVLK DGAKMSKSKG NTVDPAQMIE KYGADTVRLF 

       610        620        630        640        650        660 
ILFAAPPTQN LEWSDSGLEG VHRFINKVYR LIMDFIQDHK SHAIGTLNNF DKAQKDIRLK 

       670        680        690        700        710        720 
THQTLSKITD NMSRRYLFNT VIAALMKLCN TLSKFNDTSK TSMAIRQESI HILLKTLSPI 

       730        740        750        760        770        780 
APHLCHYLWK KLGNIKAIIN EPWPNVDKSA LVQDKMQIIV QVNGKLRTKL MFSTDVDNAQ 

       790        800        810 
IEAQTLANEN ITKFTKGKII VKVIIVPNKL VNIVVK 

« Hide

References

[1]"The Calyptogena magnifica chemoautotrophic symbiont genome."
Newton I.L.G., Woyke T., Auchtung T.A., Dilly G.F., Dutton R.J., Fisher M.C., Fontanez K.M., Lau E., Stewart F.J., Richardson P.M., Barry K.W., Saunders E., Detter J.C., Wu D., Eisen J.A., Cavanaugh C.M.
Science 315:998-1000(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000488 Genomic DNA. Translation: ABL01809.1.
RefSeqYP_903280.1. NC_008610.1.

3D structure databases

ProteinModelPortalA1AV52.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING413404.Rmag_0005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL01809; ABL01809; Rmag_0005.
GeneID4555501.
KEGGrma:Rmag_0005.
PATRIC31999537. VBICanRut45856_0005.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCRUT413404:GHM7-5-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RUTMC
AccessionPrimary (citable) accession number: A1AV52
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries