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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99ZincUniRule annotation1
Metal bindingi101ZincUniRule annotation1
Metal bindingi126ZincUniRule annotation1
Metal bindingi128ZincUniRule annotation1
Binding sitei241ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Ppro_3484
OrganismiPelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1)
Taxonomic identifieri338966 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesDesulfuromonadaceaePelobacter
Proteomesi
  • UP000006732 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000019301 – 469Glutamate--tRNA ligaseAdd BLAST469

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi338966.Ppro_3484

Structurei

3D structure databases

ProteinModelPortaliA1AUQ6
SMRiA1AUQ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionUniRule annotationAdd BLAST11
Motifi238 – 242"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A1AUQ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIRVRFAP SPTGYLHVGG ARTALFNWLL ARKQGGTFIL RIEDTDVERS
60 70 80 90 100
TQESVDAILQ GMEWLGLDWD EGPFYQTDNF PLYREYIQKL LDEGKAYRCY
110 120 130 140 150
CTAEELEAKR ELAMKEGRKP KYDGTCRDLT EQPADRPFVV RFRAPQDGGA
160 170 180 190 200
TSFNDLIKGT ITFPNDELDD LIVQRSDGTP TYNFCVVIDD AIMKITTVIR
210 220 230 240 250
GDDHVNNTPR QVQLYQALGF PVPQFAHVPM ILGSDKARLS KRHGATSVIA
260 270 280 290 300
YRDMGFLPEA LMNYLVRLGW SHGDDEIFSR DEMVAKFDIS NVGRSPSVFN
310 320 330 340 350
PDKLLWLNAH YIKHGDPQRL ADLLVPFLKM RGVDPANGGP QLAVAIKTLQ
360 370 380 390 400
ERARTMLEMA DSALFYYHAP ESYDRTALAK FEKEHLLAVY ATVAEKLSAA
410 420 430 440 450
TAVTAAEFDA LFKEICAEKG WKMPQVGQPV RIALSGGTHA PGIGEIIVTL
460
GKEETIQRIQ RAREFVEKQ
Length:469
Mass (Da):52,729
Last modified:January 23, 2007 - v1
Checksum:i94493BD6C9EFF23B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000482 Genomic DNA Translation: ABL01077.1
RefSeqiWP_011737292.1, NC_008609.1

Genome annotation databases

EnsemblBacteriaiABL01077; ABL01077; Ppro_3484
KEGGippd:Ppro_3484

Similar proteinsi

Entry informationi

Entry nameiSYE_PELPD
AccessioniPrimary (citable) accession number: A1AUQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health