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Protein

Glutamate 5-kinase

Gene

proB

Organism
Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATPUniRule annotation1
Binding sitei55SubstrateUniRule annotation1
Binding sitei142SubstrateUniRule annotation1
Binding sitei154Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 175ATPUniRule annotation2
Nucleotide bindingi216 – 222ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Ppro_2953
OrganismiPelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1)
Taxonomic identifieri338966 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesDesulfuromonadaceaePelobacter
Proteomesi
  • UP000006732 Componenti: Chromosome

Subcellular locationi

A1AT80:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000810851 – 373Glutamate 5-kinaseAdd BLAST373

Interactioni

Protein-protein interaction databases

STRINGi338966.Ppro_2953.

Structurei

3D structure databases

ProteinModelPortaliA1AT80.
SMRiA1AT80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini281 – 359PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246369.
KOiK00931.
OMAiTHEIRFG.
OrthoDBiPOG091H00OF.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR036393. AceGlu_kinase-like_sf.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR036974. PUA_sf.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.

Sequencei

Sequence statusi: Complete.

A1AT80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRELFRKIK RVVVKIGSRV LTDDEGALDM GVIGRICGDI ASLRRQGRQV
60 70 80 90 100
VLVSSGAIAA GRSELGMTEK PRTIPHKQAA AAIGQTRMMR AYEESFAPHG
110 120 130 140 150
LKVAQVLLTR EDLASRQRFL NARATLDALL GFGVIPVINE NDTVVVEEIK
160 170 180 190 200
FGDNDNLSAL VTNVAEAGLL VIMTDIEGFY SADPRSNPDA VLVPLVQGIT
210 220 230 240 250
REIERAAGGS GSSVGTGGMA TKVAAAKKAA KNGVPTIIVP GKREGIIATL
260 270 280 290 300
MAGQEVGTLF LPLDACLNRR KHWLAYSLKP AGRIIVDDGA REVLLKKGKS
310 320 330 340 350
LLPSGVLRVE GRFERGACVR VCGSDEQEFA RGLSDYSSSE IARLAGQRSS
360 370
RIEAILGYRY GDVIIHRDNL VVL
Length:373
Mass (Da):40,094
Last modified:January 23, 2007 - v1
Checksum:iD326BBC75EC1FDF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000482 Genomic DNA. Translation: ABL00551.1.
RefSeqiWP_011736786.1. NC_008609.1.

Genome annotation databases

EnsemblBacteriaiABL00551; ABL00551; Ppro_2953.
KEGGippd:Ppro_2953.

Similar proteinsi

Entry informationi

Entry nameiPROB_PELPD
AccessioniPrimary (citable) accession number: A1AT80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families