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A1APS3 (SYL_PELPD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ppro_1731
OrganismPelobacter propionicus (strain DSM 2379) [Complete proteome] [HAMAP]
Taxonomic identifier338966 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesPelobacteraceaePelobacter

Protein attributes

Sequence length823 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 823823Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009389

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1APS3 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: CAB6CCB2970CA526

FASTA82393,197
        10         20         30         40         50         60 
MEQKYNPAAV EQKWQQQWET GASFAVNEQS DRPKYYLLEM FPYPSGRIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VIGRFKRMRG FNVLHPMGWD AFGMPAENAA IQNKSHPAKW TYENIDYMRG QLKKMGLSYD 

       130        140        150        160        170        180 
WGRELATCDL EYYRWEQKIF LEMFAKGLAY KKTSYVNWCP TCETVLANEQ VEDGACWRCD 

       190        200        210        220        230        240 
SPVQQKELDQ WFFRITQYAE ELLSYTEKLP GWPERVLVMQ KNWIGKSHGC EVDFPLEGGT 

       250        260        270        280        290        300 
GSVRVFTTRP DTLFGATFMS LAPEHPQARE LTTPDRRDEV ERFIEKVRNT DRSKRTADDY 

       310        320        330        340        350        360 
EKEGVFTGSY CINPLTQVRM PIYLANFVLT DYGTGAVMAV PTHDQRDFEF AKKYGLDLKV 

       370        380        390        400        410        420 
VIQPEGTTLD PATMEAAYTE PGIMVNSGTF DGMGSEEAKQ AIADHLQEMG GGTKTVTYRL 

       430        440        450        460        470        480 
RDWGISRQRY WGNPIPVIYC DCCGVVPVPE KDLPVRLPMD VEFTGEGGSP LARLESFINV 

       490        500        510        520        530        540 
PCPTCGKPAR RETDTMDTFV QSSWYFLRYC CPDFHDAPLD RTRVDYWMSV DQYIGGIEHA 

       550        560        570        580        590        600 
VLHLLYARFF TKVLRDLGYV ECSEPFTNLL TQGMVIKDGA KMSKSKGNVV DPNALIERYG 

       610        620        630        640        650        660 
ADTARLFSLF AAPPEKDLDW NDQGVDGSFR FLNRVWKLVN DRLELISGAA PLDVPGLTGE 

       670        680        690        700        710        720 
ERTLRRAVHK TIRKVTEDIE GRFHFNTAIA AVMELLNVLQ SADLSTPQAG AVMKEALESL 

       730        740        750        760        770        780 
VLLLAPFVPH MAEELWQRMG QPGALSTTPW PEYDREAAVD DEVLMVVQVN GKLRSKITVA 

       790        800        810        820 
AGSDEESLKA LALADDKVQP FLQGVQVRKV ICVKGKLVNI VVG 

« Hide

References

[1]"Complete sequence of chromosome of Pelobacter propionicus DSM 2379."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Kim E., Lovley D., Richardson P.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2379.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000482 Genomic DNA. Translation: ABK99343.1.
RefSeqYP_901401.1. NC_008609.1.

3D structure databases

ProteinModelPortalA1APS3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING338966.Ppro_1731.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK99343; ABK99343; Ppro_1731.
GeneID4572832.
KEGGppd:Ppro_1731.
PATRIC22896557. VBIPelPro64470_1887.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycPPRO338966:GHL0-1763-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PELPD
AccessionPrimary (citable) accession number: A1APS3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries