Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pelobacter propionicus (strain DSM 2379)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPRO338966:GHL0-1763-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Ppro_1731
OrganismiPelobacter propionicus (strain DSM 2379)
Taxonomic identifieri338966 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesPelobacteraceaePelobacter
ProteomesiUP000006732 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 823823Leucine--tRNA ligasePRO_1000009389Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi338966.Ppro_1731.

Structurei

3D structure databases

ProteinModelPortaliA1APS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi581 – 5855"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1APS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQKYNPAAV EQKWQQQWET GASFAVNEQS DRPKYYLLEM FPYPSGRIHM
60 70 80 90 100
GHVRNYSIGD VIGRFKRMRG FNVLHPMGWD AFGMPAENAA IQNKSHPAKW
110 120 130 140 150
TYENIDYMRG QLKKMGLSYD WGRELATCDL EYYRWEQKIF LEMFAKGLAY
160 170 180 190 200
KKTSYVNWCP TCETVLANEQ VEDGACWRCD SPVQQKELDQ WFFRITQYAE
210 220 230 240 250
ELLSYTEKLP GWPERVLVMQ KNWIGKSHGC EVDFPLEGGT GSVRVFTTRP
260 270 280 290 300
DTLFGATFMS LAPEHPQARE LTTPDRRDEV ERFIEKVRNT DRSKRTADDY
310 320 330 340 350
EKEGVFTGSY CINPLTQVRM PIYLANFVLT DYGTGAVMAV PTHDQRDFEF
360 370 380 390 400
AKKYGLDLKV VIQPEGTTLD PATMEAAYTE PGIMVNSGTF DGMGSEEAKQ
410 420 430 440 450
AIADHLQEMG GGTKTVTYRL RDWGISRQRY WGNPIPVIYC DCCGVVPVPE
460 470 480 490 500
KDLPVRLPMD VEFTGEGGSP LARLESFINV PCPTCGKPAR RETDTMDTFV
510 520 530 540 550
QSSWYFLRYC CPDFHDAPLD RTRVDYWMSV DQYIGGIEHA VLHLLYARFF
560 570 580 590 600
TKVLRDLGYV ECSEPFTNLL TQGMVIKDGA KMSKSKGNVV DPNALIERYG
610 620 630 640 650
ADTARLFSLF AAPPEKDLDW NDQGVDGSFR FLNRVWKLVN DRLELISGAA
660 670 680 690 700
PLDVPGLTGE ERTLRRAVHK TIRKVTEDIE GRFHFNTAIA AVMELLNVLQ
710 720 730 740 750
SADLSTPQAG AVMKEALESL VLLLAPFVPH MAEELWQRMG QPGALSTTPW
760 770 780 790 800
PEYDREAAVD DEVLMVVQVN GKLRSKITVA AGSDEESLKA LALADDKVQP
810 820
FLQGVQVRKV ICVKGKLVNI VVG
Length:823
Mass (Da):93,197
Last modified:January 22, 2007 - v1
Checksum:iCAB6CCB2970CA526
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000482 Genomic DNA. Translation: ABK99343.1.
RefSeqiYP_901401.1. NC_008609.1.

Genome annotation databases

EnsemblBacteriaiABK99343; ABK99343; Ppro_1731.
KEGGippd:Ppro_1731.
PATRICi22896557. VBIPelPro64470_1887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000482 Genomic DNA. Translation: ABK99343.1.
RefSeqiYP_901401.1. NC_008609.1.

3D structure databases

ProteinModelPortaliA1APS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi338966.Ppro_1731.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK99343; ABK99343; Ppro_1731.
KEGGippd:Ppro_1731.
PATRICi22896557. VBIPelPro64470_1887.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPPRO338966:GHL0-1763-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Pelobacter propionicus DSM 2379."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F.
    , Land M., Hauser L., Kyrpides N., Kim E., Lovley D., Richardson P.
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2379.

Entry informationi

Entry nameiSYL_PELPD
AccessioniPrimary (citable) accession number: A1APS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 14, 2008
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.