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Protein

Lipoyl synthase

Gene

lipA

Organism
Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA), Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi65Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi71Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi86Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi90Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi93Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:Ppro_1029
OrganismiPelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1)
Taxonomic identifieri338966 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesDesulfuromonadaceaePelobacter
Proteomesi
  • UP000006732 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000122521 – 314Lipoyl synthaseAdd BLAST314

Interactioni

Protein-protein interaction databases

STRINGi338966.Ppro_1029

Structurei

3D structure databases

ProteinModelPortaliA1AMT5
SMRiA1AMT5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
HOGENOMiHOG000235997
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG091H069D

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

A1AMT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVQPTTMRG AVKTAHLGTD VSLATKPLPK PEWLRGKSAS TPDVERLVRI
60 70 80 90 100
LRDNRLHTVC EEASCPNLGE CFRKGTATFM IMGDVCTRHC PFCNVAHGSP
110 120 130 140 150
HELAADEPVN LARAVELLKL SYVVITSVTR DDLPDGGAGH YGACVRALRD
160 170 180 190 200
LKRSLKVEIL TPDFRGAVAV AFEELRMNLP DVFNHNLETV PRLYPRVRPQ
210 220 230 240 250
ADYHGSLDLL LRFREQFDHV PTKSGLMLGL GETEQEVRDV MEELRRHRCD
260 270 280 290 300
MLTLGQYMRP SPHHLPVERY VTPDEFERYR QFGLSIGFSH VESGPMVRSS
310
YHADMQAREL MLVN
Length:314
Mass (Da):35,484
Last modified:January 23, 2007 - v1
Checksum:i222D9BADED7A669D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000482 Genomic DNA Translation: ABK98655.1
RefSeqiWP_011734959.1, NC_008609.1

Genome annotation databases

EnsemblBacteriaiABK98655; ABK98655; Ppro_1029
KEGGippd:Ppro_1029

Similar proteinsi

Entry informationi

Entry nameiLIPA_PELPD
AccessioniPrimary (citable) accession number: A1AMT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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