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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 14FADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandFAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:Ppro_0527
OrganismiPelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1)
Taxonomic identifieri338966 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesDesulfuromonadaceaePelobacter
Proteomesi
  • UP000006732 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003463721 – 454Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST454

Interactioni

Protein-protein interaction databases

STRINGi338966.Ppro_0527.

Structurei

3D structure databases

ProteinModelPortaliA1ALD8.
SMRiA1ALD8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
PANTHERiPTHR11806. PTHR11806. 1 hit.
PTHR11806:SF5. PTHR11806:SF5. 1 hit.
PfamiView protein in Pfam
PF01134. GIDA. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

A1ALD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHSLTIIGA GLAGCEAAWQ AARRGVSVTL HEMKPEKRSP AHHLPGLAEL
60 70 80 90 100
VCSNSLRGDS LENAVGLLKE ELRRGGSLIM EAAEATRVPA GGALAVDRQL
110 120 130 140 150
FSDYVTAKIT AHPLINLVHG EMDAIPAEGT VIIASGPLTS DALAASLSGL
160 170 180 190 200
TGDRLYFYDS IAPIVTADSL DREKVFAASR YGKGDGDDYL NCPLDREQYQ
210 220 230 240 250
AFVEQLNRGE KVAAREFEKL VHFEGCMPVE ELAGRGEDTL RFGPMKPVGL
260 270 280 290 300
TDPRTGIEPH AVIQLRAENR EKTMYNLVGF QTKLTWPEQR RIFRTIPGLE
310 320 330 340 350
RAEFVRLGSM HRNTFINAPA LLQPTQQLRS DPRIFFAGQI TGVEGYVESA
360 370 380 390 400
GSGFLAGITA ARLLTGMPDP AVPPAETALG ALVSHITNAD VRHFQPMNVN
410 420 430 440 450
YGLFPDLEGR IKKKERRGKL AERALASLSG WLERVSPPSR ETGEPTGAEQ

VDLA
Length:454
Mass (Da):49,292
Last modified:January 23, 2007 - v1
Checksum:i5927CE628D2CA15A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000482 Genomic DNA. Translation: ABK98158.1.
RefSeqiWP_011734471.1. NC_008609.1.

Genome annotation databases

EnsemblBacteriaiABK98158; ABK98158; Ppro_0527.
KEGGippd:Ppro_0527.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRMFO_PELPD
AccessioniPrimary (citable) accession number: A1ALD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families