A1ALC4 (FMT_PELPD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	Ppro_0513

Organism

Pelobacter propionicus (strain DSM 2379) [Complete proteome] [HAMAP]

Taxonomic identifier

338966 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfuromonadales › Pelobacteraceae › Pelobacter › Pelobacter propionicus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000020120
Regions
	Region	112 – 115	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1ALC4 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 26D0863B8A86E267	FASTA	319	34,234
	10 20 30 40 50 60 MSSRSIVFMG TPEFACPTLQ ALIDRGERLL AVVTQPDRPK GRGHKLMPPP VKELALAHDI 70 80 90 100 110 120 PVLQPHRVRA SAFVESIRQL APELIVVVAF GQILPKALLD IPPLGCVNVH ASLLPRYRGA 130 140 150 160 170 180 APLNWCIING ETETGVTTML MDTGLDTGPM LLKRSTPIDE NEDIVSLHDR MASLGAELLA 190 200 210 220 230 240 ETLDGLREGR IEPQPQNDSL SCYAPLLKKE HGLIDWQRPA RQIHNQVRGL AAWPGAQTLL 250 260 270 280 290 300 NGHALKLFRT RVADGAGAPG TVLCSTGGQL EVACLDGSLL IQELQLAGKK RLDSASFLAG 310 CPIAEGTLLG GAATERPIP

References

[1]

"Complete sequence of chromosome of Pelobacter propionicus DSM 2379." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Lovley D., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2379.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000482 Genomic DNA. Translation: ABK98144.1.
RefSeq	YP_900202.1. NC_008609.1.
3D structure databases
ProteinModelPortal	A1ALC4.
ModBase	Search...
Protein-protein interaction databases
STRING	338966.Ppro_0513.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK98144; ABK98144; Ppro_0513.
GeneID	4572884.
KEGG	ppd:Ppro_0513.
PATRIC	22894063. VBIPelPro64470_0670.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	DWNKSAR.
ProtClustDB	CLSK827657.
Enzyme and pathway databases
BioCyc	PPRO338966:GHL0-580-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_PELPD

Accession

Primary (citable) accession number: A1ALC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program