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Protein

Glycerol-3-phosphate acyltransferase

Gene

plsB

Organism
Escherichia coli O1:K1 / APEC
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.UniRule annotation

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. no protein annotated in this organism
  3. Phosphatidate cytidylyltransferase (cdsA)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferaseUniRule annotation (EC:2.3.1.15UniRule annotation)
Short name:
GPATUniRule annotation
Gene namesi
Name:plsBUniRule annotation
Ordered Locus Names:Ecok1_40140
ORF Names:APECO1_2428
OrganismiEscherichia coli O1:K1 / APEC
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008216 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000494301 – 827Glycerol-3-phosphate acyltransferaseAdd BLAST827

Proteomic databases

PRIDEiA1AIL8.

Structurei

3D structure databases

ProteinModelPortaliA1AIL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi325 – 330HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.UniRule annotation

Sequence similaritiesi

Belongs to the GPAT/DAPAT family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans. 1 hit.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1AIL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFCYPCRAF ALLTRGFTSF MSGWPRIYYK LLNLPLSILV KSKSIPADPA
60 70 80 90 100
PELGLDTSRP IMYVLPYNSK ADLLTLRAQC LAHDLPDPLE PLEIDGTLLP
110 120 130 140 150
RYVFIHGGPR VFTYYTPKEE SIKLFHDYLD LHRSNPNLDV QMVPVSVMFG
160 170 180 190 200
RAPGREKGEV NPPLRMLNGV QKFFAVLWLG RDSFVRFSPS VSLRRMADEH
210 220 230 240 250
GTDKTIAQKL ARVARMHFAR QRLAAVGPRL PARQDLFNKL LASRAIAKAV
260 270 280 290 300
EDEARSKKIS HEKAQQNAIA LMEEIAANFS YEMIRLTDRI LGFTWNRLYQ
310 320 330 340 350
GINVHNAERV RQLAHDGHEL VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA
360 370 380 390 400
AGINLNFWPA GPIFRRLGAF FIRRTFKGNK LYSTVFREYL GELFSRGYSV
410 420 430 440 450
EYFVEGGRSR TGRLLDPKTG TLSMTIQAML RGGTRPITLI PIYIGYEHVM
460 470 480 490 500
EVGTYAKELR GATKEKESLP QMLRGLSKLR NLGQGYVNFG EPMPLMTYLN
510 520 530 540 550
QHVPDWRESI DPIEAVRPAW LTPTVNNIAA DLMVRINNAG AANAMNLCCT
560 570 580 590 600
ALLASRQRSL TREQLTEQLN CYLDLMRNVP YSTDSTVPSA SASELIDHAL
610 620 630 640 650
QMNKFEVEKD TIGDIIILPR EQAVLMTYYR NNIAHMLVLP SLMAAIVTQH
660 670 680 690 700
RHISRDVLME HVNVLYPMLK AELFLRWDRD ELPDVIDALA NEMQRQGLIT
710 720 730 740 750
LQDDELHINP AHSRTLQLLA AGARETLQRY AITFWLLSAN PSINRGTLEK
760 770 780 790 800
ESRTVAQRLS VLHGINAPEF FDKAVFSSLV LTLRDEGYIS DSGDAEPAET
810 820
MKVYQLLAEL ITSDVRLTIE SATQGEG
Length:827
Mass (Da):93,696
Last modified:January 23, 2007 - v1
Checksum:i30DF48AC985EB183
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ03508.1.

Genome annotation databases

EnsemblBacteriaiABJ03508; ABJ03508; APECO1_2428.
KEGGiecv:APECO1_2428.
PATRICi18220637. VBIEscCol127180_4516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ03508.1.

3D structure databases

ProteinModelPortaliA1AIL8.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiA1AIL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ03508; ABJ03508; APECO1_2428.
KEGGiecv:APECO1_2428.
PATRICi18220637. VBIEscCol127180_4516.

Phylogenomic databases

HOGENOMiHOG000218231.
KOiK00631.
OMAiEVIYVPC.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00612.

Family and domain databases

HAMAPiMF_00393. Glyc3P_acyltrans. 1 hit.
InterProiIPR022284. GPAT/DHAPAT.
IPR028354. GPAT_PlsB.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PANTHERiPTHR12563. PTHR12563. 2 hits.
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
PIRSFiPIRSF500064. GPAT. 1 hit.
PIRSF000437. GPAT_DHAPAT. 1 hit.
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03703. plsB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLSB_ECOK1
AccessioniPrimary (citable) accession number: A1AIL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.