Reviewed, UniProtKB/Swiss-Prot A1AIL8 (PLSB_ECOK1)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Ecok1_40140
ORF Names:	APECO1_2428

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049430
Regions
	Motif	325 – 330	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A1AIL8-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 30DF48AC985EB183	FASTA	827	93,696
	10 20 30 40 50 60 MTFCYPCRAF ALLTRGFTSF MSGWPRIYYK LLNLPLSILV KSKSIPADPA PELGLDTSRP 70 80 90 100 110 120 IMYVLPYNSK ADLLTLRAQC LAHDLPDPLE PLEIDGTLLP RYVFIHGGPR VFTYYTPKEE 130 140 150 160 170 180 SIKLFHDYLD LHRSNPNLDV QMVPVSVMFG RAPGREKGEV NPPLRMLNGV QKFFAVLWLG 190 200 210 220 230 240 RDSFVRFSPS VSLRRMADEH GTDKTIAQKL ARVARMHFAR QRLAAVGPRL PARQDLFNKL 250 260 270 280 290 300 LASRAIAKAV EDEARSKKIS HEKAQQNAIA LMEEIAANFS YEMIRLTDRI LGFTWNRLYQ 310 320 330 340 350 360 GINVHNAERV RQLAHDGHEL VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA 370 380 390 400 410 420 GPIFRRLGAF FIRRTFKGNK LYSTVFREYL GELFSRGYSV EYFVEGGRSR TGRLLDPKTG 430 440 450 460 470 480 TLSMTIQAML RGGTRPITLI PIYIGYEHVM EVGTYAKELR GATKEKESLP QMLRGLSKLR 490 500 510 520 530 540 NLGQGYVNFG EPMPLMTYLN QHVPDWRESI DPIEAVRPAW LTPTVNNIAA DLMVRINNAG 550 560 570 580 590 600 AANAMNLCCT ALLASRQRSL TREQLTEQLN CYLDLMRNVP YSTDSTVPSA SASELIDHAL 610 620 630 640 650 660 QMNKFEVEKD TIGDIIILPR EQAVLMTYYR NNIAHMLVLP SLMAAIVTQH RHISRDVLME 670 680 690 700 710 720 HVNVLYPMLK AELFLRWDRD ELPDVIDALA NEMQRQGLIT LQDDELHINP AHSRTLQLLA 730 740 750 760 770 780 AGARETLQRY AITFWLLSAN PSINRGTLEK ESRTVAQRLS VLHGINAPEF FDKAVFSSLV 790 800 810 820 LTLRDEGYIS DSGDAEPAET MKVYQLLAEL ITSDVRLTIE SATQGEG

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed: 17293413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000468 Genomic DNA. Translation: ABJ03508.1.
RefSeq	YP_859632.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1AIL8.
Genome annotation databases
GeneID	4493032.
GenomeReviews	Gene locus Ecok1_40140 in contig CP000468_GR.
KEGG	ecv:APECO1_2428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VPVYIGY.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_ECOK1

Accession

Primary (citable) accession number: A1AIL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)