A1AI83 (RHAR_ECOK1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional activator RhaR

Alternative name(s):
L-rhamnose operon transcriptional activator RhaR

Gene names

Name:	rhaR
Ordered Locus Names:	Ecok1_38790
ORF Names:	APECO1_2562

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates expression of the rhaSR operon in response to L-rhamnose By similarity. HAMAP-Rule MF_01533
Subunit structure	Binds DNA as a dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 2 HTH araC/xylS-type DNA-binding domains.
Sequence caution	The sequence ABJ03373.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Rhamnose metabolism Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Repeat
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP rhamnose metabolic process Inferred from electronic annotation. Source: HAMAP transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	HTH-type transcriptional activator RhaR HAMAP-Rule MF_01533		PRO_0000292772
Regions
	DNA binding	195 – 214	20	H-T-H motif By similarity
	DNA binding	244 – 267	24	H-T-H motif By similarity
Sites
	Site	246	1	Interaction with sigma-70 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1AI83 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 48DC6942F46DBC88	FASTA	282	32,341
	10 20 30 40 50 60 MAHQLKLLKD DFFASDQQAV AVADRYPQDV FAEHTHDFCE LVIVWRGNGL HVLNDRPYRI 70 80 90 100 110 120 TRGDLFYIHA DDKHSYASVN DLVLQNIIYC PERLKLNLDW QGAIPGFNAS AGQPHWRLGS 130 140 150 160 170 180 VGMAQARQVI GQLEHESSQH VPFANEMAEL LFGQLVMLLN RHRYTSDSLP PTSSETLLDK 190 200 210 220 230 240 LITRLAASLK SPFALDKFCD EASCSERVLR QQFRQQTGMT INQYLRQVRV CHAQYLLQHS 250 260 270 280 RLLISDISTE CGFEDSNYFS VVFTRETGMT PSQWRHLNSQ KD

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000468 Genomic DNA. Translation: ABJ03373.1. Different initiation.
RefSeq	YP_859497.1. NC_008563.1.
3D structure databases
ProteinModelPortal	A1AI83.
SMR	A1AI83. Positions 24-89, 175-278.
ModBase	Search...
Protein-protein interaction databases
STRING	405955.APECO1_2562.
Protein family/group databases
AraC-XylS	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ03373; ABJ03373; APECO1_2562.
GeneID	4495588.
KEGG	ecv:APECO1_2562.
PATRIC	18220327. VBIEscCol127180_4373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2207.
HOGENOM	HOG000290513.
KO	K02854.
ProtClustDB	PRK13500.
Family and domain databases
Gene3D	1.10.10.60. 2 hits.
HAMAP	MF_01533. HTH-type_RhaR.
InterPro	IPR009057. Homeodomain-like. IPR018060. HTH_AraC. IPR018062. HTH_AraC-typ_CS. IPR011051. RmlC_Cupin. IPR023699. Tscrpt_act_RhaR. IPR003313. Tscrpt_reg_HTH_AraC-bd. IPR020449. Tscrpt_reg_HTH_AraC-type. [Graphical view]
Pfam	PF02311. AraC_binding. 1 hit. PF12833. HTH_18. 1 hit. [Graphical view]
PRINTS	PR00032. HTHARAC.
SMART	SM00342. HTH_ARAC. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 2 hits. SSF51182. RmlC_like_cupin. 1 hit.
PROSITE	PS00041. HTH_ARAC_FAMILY_1. 1 hit. PS01124. HTH_ARAC_FAMILY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RHAR_ECOK1

Accession

Primary (citable) accession number: A1AI83

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	July 24, 2007
Last modified:	May 1, 2013
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program