A1AI82 (RHAS_ECOK1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional activator RhaS

Alternative name(s):
L-rhamnose operon regulatory protein RhaS

Gene names

Name:	rhaS
Ordered Locus Names:	Ecok1_38780
ORF Names:	APECO1_2563

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates expression of the rhaBAD and rhaT operons By similarity. HAMAP-Rule MF_01534
Subunit structure	Binds DNA as a dimer By similarity. HAMAP-Rule MF_01534
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01534.
Sequence similarities	Contains 2 HTH araC/xylS-type DNA-binding domains.

Ontologies

Keywords
Biological process	Rhamnose metabolism Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Repeat
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP rhamnose metabolic process Inferred from electronic annotation. Source: HAMAP transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	HTH-type transcriptional activator RhaS HAMAP-Rule MF_01534		PRO_1000068702
Regions
	DNA binding	190 – 209	20	H-T-H motif By similarity
	DNA binding	239 – 262	24	H-T-H motif By similarity
Sites
	Site	241	1	Interaction with sigma-70 By similarity
	Site	250	1	Interaction with sigma-70 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1AI82 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 71E981876A6F21A9	FASTA	278	32,402
	10 20 30 40 50 60 MTVLHSVDFF PSGNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF NGQPYTITGG 70 80 90 100 110 120 TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ LLPQEQDGQY PSHWRVNHSV 130 140 150 160 170 180 LQQVRQLVAQ MEQQEEENDL PSTASREILF MQLLLLLRKS SLQENLENSA SRLNLLLAWL 190 200 210 220 230 240 EDHFADEVNW DAVADQFSLS LRTLHRQLKQ KTGLTPQRYL NRLRLMKARH LLRHSEASVT 250 260 270 DIAYRCGFSD SNHFSTLFRR EFNWSPRDIR QGRDGFLQ

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000468 Genomic DNA. Translation: ABJ03372.1.
RefSeq	YP_859496.1. NC_008563.1.
3D structure databases
ProteinModelPortal	A1AI82.
SMR	A1AI82. Positions 22-83, 170-274.
ModBase	Search...
Protein-protein interaction databases
STRING	405955.APECO1_2563.
Protein family/group databases
AraC-XylS	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ03372; ABJ03372; APECO1_2563.
GeneID	4495587.
KEGG	ecv:APECO1_2563.
PATRIC	18220325. VBIEscCol127180_4372.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2207.
HOGENOM	HOG000290513.
KO	K02855.
OMA	RYLYEHT.
ProtClustDB	PRK13503.
Family and domain databases
Gene3D	1.10.10.60. 2 hits. 2.60.120.10. 1 hit.
HAMAP	MF_01534. HTH-type_RhaS.
InterPro	IPR009057. Homeodomain-like. IPR018060. HTH_AraC. IPR018062. HTH_AraC-typ_CS. IPR014710. RmlC-like_jellyroll. IPR003313. Tscrpt_reg_HTH_AraC-bd. IPR020449. Tscrpt_reg_HTH_AraC-type. IPR023609. Tscrpt_reg_HTH_RhaS. [Graphical view]
Pfam	PF02311. AraC_binding. 1 hit. PF12833. HTH_18. 1 hit. [Graphical view]
PRINTS	PR00032. HTHARAC.
SMART	SM00342. HTH_ARAC. 1 hit. [Graphical view]
SUPFAM	SSF51215. AraC_binding. 1 hit. SSF46689. Homeodomain_like. 2 hits.
PROSITE	PS00041. HTH_ARAC_FAMILY_1. 1 hit. PS01124. HTH_ARAC_FAMILY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RHAS_ECOK1

Accession

Primary (citable) accession number: A1AI82

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program