A1AHH2 (SLMA_ECOK1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoid occlusion factor SlmA

Gene names

Name:	slmA
Ordered Locus Names:	Ecok1_36180
ORF Names:	APECO1_2820

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions By similarity. HAMAP-Rule MF_01839
Subunit structure	Homodimer By similarity. Interacts with FtsZ By similarity.
Subcellular location	Cytoplasm › nucleoid By similarity.
Sequence similarities	Belongs to the nucleoid occlusion factor SlmA family. Contains 1 HTH tetR-type DNA-binding domain.
Sequence caution	The sequence ABJ03112.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of barrier septum assembly Inferred from electronic annotation. Source: InterPro
Cellular_component	bacterial nucleoid Inferred from electronic annotation. Source: HAMAP cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Nucleoid occlusion factor SlmA HAMAP-Rule MF_01839		PRO_0000413758
Regions
	Domain	10 – 70	61	HTH tetR-type
	DNA binding	33 – 52	20	H-T-H motif By similarity
	Coiled coil	117 – 144	28	Potential

Sequences

	Sequence		Length	Mass (Da)
	A1AHH2 [UniParc]. Last modified November 16, 2011. Version 2. Checksum: C78D00E2875B1F39	FASTA	198	22,806
	10 20 30 40 50 60 MAEKQTAKRN RREEILQSLA LMLESSDGSQ RITTAKLAAS VGVSEAALYR HFPSKTRMFD 70 80 90 100 110 120 SLIEFIEDSL ITRINLILKD EKDTTARLRL IVLLLLGFGE RNPGLTRILT GHALMFEQDR 130 140 150 160 170 180 LQGRINQLFE RIEAQLRQVL REKRMREGEG YATDETLLAS QILAFCEGML SRFVRSEFKY 190 RPTDDFDARW PLIAAQLQ

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000468 Genomic DNA. Translation: ABJ03112.1. Different initiation.
RefSeq	YP_859236.1. NC_008563.1.
3D structure databases
ProteinModelPortal	A1AHH2.
SMR	A1AHH2. Positions 10-111.
ModBase	Search...
Protein-protein interaction databases
STRING	405955.APECO1_2820.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ03112; ABJ03112; APECO1_2820.
GeneID	4492600.
KEGG	ecv:APECO1_2820.
PATRIC	18219727. VBIEscCol127180_4089.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1309.
HOGENOM	HOG000266053.
KO	K05501.
ProtClustDB	PRK09480.
Family and domain databases
Gene3D	1.10.357.10. 1 hit.
HAMAP	MF_01839. NO_factor_SlmA.
InterPro	IPR023772. DNA-bd_HTH_TetR-type_CS. IPR009057. Homeodomain-like. IPR001647. HTH_TetR. IPR023769. NO_SlmA. IPR015893. Tet_transcr_reg_TetR-like_C. IPR011075. Tet_transcr_reg_TetR-rel_C. [Graphical view]
Pfam	PF00440. TetR_N. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit. SSF48498. TetR_like_C. 1 hit.
PROSITE	PS01081. HTH_TETR_1. 1 hit. PS50977. HTH_TETR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SLMA_ECOK1

Accession

Primary (citable) accession number: A1AHH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2011
Last sequence update:	November 16, 2011
Last modified:	May 1, 2013
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program