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Protein

Ribosomal RNA small subunit methyltransferase B

Gene

rsmB

Organism
Escherichia coli O1:K1 / APEC
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei277S-adenosyl-L-methionineUniRule annotation1
Binding sitei303S-adenosyl-L-methionineUniRule annotation1
Binding sitei322S-adenosyl-L-methionineUniRule annotation1
Active sitei375NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase BUniRule annotation (EC:2.1.1.176UniRule annotation)
Alternative name(s):
16S rRNA m5C967 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmBUniRule annotation
Gene namesi
Name:rsmBUniRule annotation
Synonyms:sunUniRule annotation
Ordered Locus Names:Ecok1_32760
ORF Names:APECO1_3158
OrganismiEscherichia coli O1:K1 / APEC
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008216 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003661571 – 429Ribosomal RNA small subunit methyltransferase BAdd BLAST429

Structurei

3D structure databases

ProteinModelPortaliA1AGI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 260S-adenosyl-L-methionine bindingUniRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000037300.
KOiK03500.
OMAiLRVNRQH.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_01856. 16SrRNA_methyltr_B. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1AGI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKQRNLRSM AAQAIEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG
60 70 80 90 100
VLRTLSQLDW LINKLMARPM TGKQRTVHYL IMVGLYQLLY TRIPPHAALA
110 120 130 140 150
ETVEGAVAIK RPQLKGLING VLRQFQRQQD ELLAEFNASD ARYLHPSWLL
160 170 180 190 200
KRLQKAYPEQ WQSIVEANNQ RPPMWLRVNR THHSRDSWLA LLDEAGMKGF
210 220 230 240 250
PHADYPDAVQ LETPAPVHAL PGFEEGWVTV QDASAQGCMT WLAPQNGEHI
260 270 280 290 300
LDLCAAPGGK TTHILEVAPE AQVLAVDIDE QRLSRVYDNL KRLGMKATVK
310 320 330 340 350
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA
360 370 380 390 400
QLQSEILDAI WSHLKSGGTL VYATCSMLPE ENSLQIKAFL QRTADAELCE
410 420
TGTPEQPGKQ NLPGAEEGDG FFYAKLIKK
Length:429
Mass (Da):48,327
Last modified:January 23, 2007 - v1
Checksum:i57845B0D89F620B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ02770.1.
RefSeqiWP_000744766.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABJ02770; ABJ02770; APECO1_3158.
KEGGiecv:APECO1_3158.
PATRICi18218948. VBIEscCol127180_3702.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ02770.1.
RefSeqiWP_000744766.1. NC_008563.1.

3D structure databases

ProteinModelPortaliA1AGI0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ02770; ABJ02770; APECO1_3158.
KEGGiecv:APECO1_3158.
PATRICi18218948. VBIEscCol127180_3702.

Phylogenomic databases

HOGENOMiHOG000037300.
KOiK03500.
OMAiLRVNRQH.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_01856. 16SrRNA_methyltr_B. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR004573. rRNA_ssu_MeTfrase_B.
IPR023541. rRNA_ssu_MeTfrase_B_ent.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00563. rsmB. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMB_ECOK1
AccessioniPrimary (citable) accession number: A1AGI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.