A1AG91 (MURA_ECOK1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
EC=2.5.1.7

Alternative name(s):
Enoylpyruvate transferase
UDP-N-acetylglucosamine enolpyruvyl transferase
Short name=EPT

Gene names

Name:	murA
Ordered Locus Names:	Ecok1_31870
ORF Names:	APECO1_3245

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	UDP-N-acetylglucosamine 1-carboxyvinyltransferase HAMAP MF_00111		PRO_1000023033
Sites
	Active site	115	1	Proton donor By similarity
Amino acid modifications
	Modified residue	115	1	2-(S-cysteinyl)pyruvic acid O-phosphothioketal By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1AG91 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 6B75A842255E53F7	FASTA	419	44,818
	10 20 30 40 50 60 MDKFRVQGPT KLQGEVTISG AKNAALPILF AALLAEEPVE IQNVPKLKDV DTSMKLLSQL 70 80 90 100 110 120 GAKVERNGSV HIDARDVNVF CAPYDLVKTM RASIWALGPL VARFGQGQVS LPGGCTIGAR 130 140 150 160 170 180 PVDLHISGLE QLGATIKLEE GYVKASVDGR LKGAHIVMDK VSVGATVTIM CAATLAEGTT 190 200 210 220 230 240 IIENAAREPE IVDTANFLIT LGAKISGQGT DRIVIEGVER LGGGVYRVLP DRIETGTFLV 250 260 270 280 290 300 AAAISRGKII CRNAQPDTLD AVLAKLRDAG ADIEVGEDWI SLDMHGKRPK AVNVRTAPHP 310 320 330 340 350 360 AFPTDMQAQF TLLNLVAEGT GFITETVFEN RFMHVPELSR MGAHAEIESN TVICHGVEKL 370 380 390 400 410 SGAQVMATDL RASASLVLAG CIAEGTTVVD RIYHIDRGYE RIEDKLRALG ANIERVKGE

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed: 17293413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000468 Genomic DNA. Translation: ABJ02681.1.
RefSeq	YP_858805.1. NC_008563.1.
3D structure databases
ProteinModelPortal	A1AG91.
SMR	A1AG91. Positions 1-419.
ModBase	Search...
Protein-protein interaction databases
STRING	A1AG91.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000039373; EBESCP00000037890; EBESCG00000038423.
GeneID	4491337.
GenomeReviews	Gene locus Ecok1_31870 in contig CP000468_GR.
KEGG	ecv:APECO1_3245.
PATRIC	18218741. VBIEscCol127180_3606.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0766.
GeneTree	EBGT00050000011541.
HOGENOM	HBG482701.
OMA	GYEHIED.
ProtClustDB	PRK09369.
Enzyme and pathway databases
BioCyc	ECOL405955:APECO1_3245-MONOMER.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00790.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01072. MurA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA_ECOK1

Accession

Primary (citable) accession number: A1AG91

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program