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Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Escherichia coli O1:K1 / APEC
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431SubstrateUniRule annotation
Active sitei48 – 481NucleophileUniRule annotation
Binding sitei76 – 761SubstrateUniRule annotation
Binding sitei179 – 1791SubstrateUniRule annotation
Binding sitei200 – 2001SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
tRNA pseudouridine(55) synthaseUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:Ecok1_31670
ORF Names:APECO1_3264
OrganismiEscherichia coli O1:K1 / APEC
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008216 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314tRNA pseudouridine synthase BPRO_1000084585Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA1AG71.
SMRiA1AG71. Positions 10-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiEOG6358D3.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09157. TruB-C_2. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1AG71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPRRRGRD INGVLLLDKP QGMSSNDALQ KVKRIYNANR AGHTGALDPL
60 70 80 90 100
ATGMLPICLG EATKFSQYLL DSDKRYRVIA RLGQRTDTSD ADGQIVEERP
110 120 130 140 150
VTFSAEQLAA ALDTFRGDIE QIPSMYSALK YQGKKLYEYA RQGIEVPREA
160 170 180 190 200
RPITVYELLF IRHEGNELEL EIHCSKGTYI RTIIDDLGEK LGCGAHVIYL
210 220 230 240 250
RRLAVSKYPV ERMVTLEHLR ELVEQAEQQD IPAAELLDPL LMPMDSPASD
260 270 280 290 300
YPVVNLPLTS SVYFKNGNPV RTSGAPLEGL VRVTEGENGK FIGMGEIDDE
310
GRVAPRRLVV EYPA
Length:314
Mass (Da):35,087
Last modified:January 23, 2007 - v1
Checksum:iFEA46C01E0E8E7A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ02661.1.
RefSeqiWP_000089698.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABJ02661; ABJ02661; APECO1_3264.
KEGGiecv:APECO1_3264.
PATRICi18218695. VBIEscCol127180_3585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ02661.1.
RefSeqiWP_000089698.1. NC_008563.1.

3D structure databases

ProteinModelPortaliA1AG71.
SMRiA1AG71. Positions 10-311.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ02661; ABJ02661; APECO1_3264.
KEGGiecv:APECO1_3264.
PATRICi18218695. VBIEscCol127180_3585.

Phylogenomic databases

HOGENOMiHOG000231223.
KOiK03177.
OMAiYELQFIR.
OrthoDBiEOG6358D3.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
HAMAPiMF_01080. TruB_bact.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09157. TruB-C_2. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes."
    Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.
    J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiTRUB_ECOK1
AccessioniPrimary (citable) accession number: A1AG71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.