Reviewed, UniProtKB/Swiss-Prot A1AG02 (RLMG_ECOK1)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	Ecok1_30980
ORF Names:	APECO1_3334

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366462

Sequences

	Sequence		Length	Mass (Da)
	A1AG02-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 0EA9DDBA96026F4A	FASTA	378	42,310
	10 20 30 40 50 60 MSHLDNGFRS LTLQRFPATD DVNPLQAWEA ADEYLLQQLD DTEIRGPVLI LNDAFGALSC 70 80 90 100 110 120 ALAEHKPYSI GDSYISELAT RENLRLNGID ESSVKFLDST ADYPQQPGVV LIKVPKTLAL 130 140 150 160 170 180 LEQQLRALRK VVTPDTRIIA GAKARDIHTS TLELFEKVLG PTTTTLAWKK ARLINCTFNE 190 200 210 220 230 240 PPLADAPQTV SWKLEGTDWT IHNHANVFSR TGLDIGARFF MQHLPENLEG EIVDLGCGNG 250 260 270 280 290 300 VIGLTLLDKN PQAKVVFVDE SPMAVASSRL NVETNMPEAL DRCEFMINNA LSGVEPFRFN 310 320 330 340 350 360 AVLCNPPFHQ QHALTDNVAW EMFHHARRCL KINGELYIVA NRHLDYFHKL KKIFGNCTTI 370 ATNNKFVVLK AVKLGRRR

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed: 17293413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000468 Genomic DNA. Translation: ABJ02592.1.
RefSeq	YP_858716.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1AG02.
Genome annotation databases
GeneID	4494991.
GenomeReviews	Gene locus Ecok1_30980 in contig CP000468_GR.
KEGG	ecv:APECO1_3334.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NRHLGYH.
Family and domain databases
HAMAP	MF_01859. [Tree]
InterPro	IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_ECOK1

Accession

Primary (citable) accession number: A1AG02

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)