A1AFC5 (SPEB_ECOK1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Agmatinase
EC=3.5.3.11

Alternative name(s):
Agmatine ureohydrolase
Short name=AUH

Gene names

Name:	speB
Ordered Locus Names:	Ecok1_28710
ORF Names:	APECO1_3592

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of putrescine from agmatine By similarity. HAMAP MF_01418
Catalytic activity	Agmatine + H2O = putrescine + urea. HAMAP MF_01418
Cofactor	Manganese By similarity. HAMAP MF_01418
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from agmatine: step 1/1. HAMAP MF_01418
Sequence similarities	Belongs to the arginase family. Agmatinase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Putrescine biosynthesis Spermidine biosynthesis
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	putrescine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW spermidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	agmatinase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Agmatinase HAMAP MF_01418		PRO_1000024278
Sites
	Metal binding	126	1	Manganese By similarity
	Metal binding	149	1	Manganese By similarity
	Metal binding	151	1	Manganese By similarity
	Metal binding	153	1	Manganese By similarity
	Metal binding	230	1	Manganese By similarity
	Metal binding	232	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1AFC5 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 5B98B07164854B9F	FASTA	306	33,571
	10 20 30 40 50 60 MSTLGHQYDN SLVSNAFGFL RLPMNFQPYD SDADWVITGV PFDMATSGRA GGRHGPAAIR 70 80 90 100 110 120 QVSTNLAWEH NRFPWNFDMR ERLNVVDCGD LVYAFGDARE MSEKLQAHAE KLLAAGKRML 130 140 150 160 170 180 SFGGDHFVTL PLLRAHAKHF GKMALVHFDA HTDTYANGCE FDHGTMFYTA PKEGLIDPNH 190 200 210 220 230 240 SVQIGIRTEF DKDNGFTVLD ACQVNDRSVD DIIAQVKQIV GDMPVYLTFD IDCLDPAFAP 250 260 270 280 290 300 GTGTPVIGGL TSDRAIKLVR GLKDLNIVGM DVVEVAPAYD QSEITALAAA TLALEMLYIQ AAKKGE

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed: 17293413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000468 Genomic DNA. Translation: ABJ02365.1.
RefSeq	YP_854154.1. NC_008563.1.
3D structure databases
ProteinModelPortal	A1AFC5.
SMR	A1AFC5. Positions 12-303.
ModBase	Search...
Protein-protein interaction databases
STRING	A1AFC5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000039607; EBESCP00000038124; EBESCG00000038657.
GeneID	4495172.
GenomeReviews	Gene locus Ecok1_28710 in contig CP000468_GR.
KEGG	ecv:APECO1_3592.
PATRIC	18218047. VBIEscCol127180_3263.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0010.
GeneTree	EBGT00050000009564.
HOGENOM	HBG391953.
OMA	IRTEYDH.
ProtClustDB	PRK02190.
Enzyme and pathway databases
BioCyc	ECOL405955:APECO1_3592-MONOMER.
Family and domain databases
HAMAP	MF_01418. SpeB. [Tree]
InterPro	IPR023694. Agmatinase. IPR005925. Agmatinase-rel. IPR006035. Ureohydrolase. IPR023696. Ureohydrolase_domain. IPR020855. Ureohydrolase_Mn_BS. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
KO	K01480.
PANTHER	PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PIRSF	PIRSF036979. Arginase. 1 hit.
TIGRFAMs	TIGR01230. Agmatinase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEB_ECOK1

Accession

Primary (citable) accession number: A1AFC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program