A1AFB0 (PGK_ECOK1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	Ecok1_28560
ORF Names:	APECO1_3607

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity. HAMAP MF_00145
Subcellular location	Cytoplasm Potential HAMAP MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Phosphoglycerate kinase HAMAP MF_00145		PRO_1000057984
Regions
	Nucleotide binding	340 – 343	4	ATP By similarity
	Region	21 – 23	3	Substrate binding By similarity
	Region	59 – 62	4	Substrate binding By similarity
Sites
	Binding site	36	1	Substrate By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	197	1	ATP By similarity
	Binding site	314	1	ATP By similarity
Amino acid modifications
	Modified residue	84	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1AFB0 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: FAD7D66D100514B2	FASTA	387	41,118
	10 20 30 40 50 60 MSVIKMTDLD LAGKRVFIRA DLNVPVKDGK VTSDARIRAS LPTIELALKQ GAKVMVTSHL 70 80 90 100 110 120 GRPTEGEYNE EFSLLPVVNY LKDKLSNPVR LVKDYLDGVD VAEGELVVLE NVRFNKGEKK 130 140 150 160 170 180 DDETLSKKYA ALCDVFVMDA FGTAHRAQAS THGIGKFADV ACAGPLLAAE LDALGKALKE 190 200 210 220 230 240 PARPMVAIVG GSKVSTKLTV LDSLSKIADQ LIVGGGIANT FIAAQGHDVG KSLYEADLVD 250 260 270 280 290 300 EAKRLLTTCN IPVPSDVRVA TEFSETAPAT LKSVNDVKAD EQILDIGDAS AQELAEILKN 310 320 330 340 350 360 AKTILWNGPV GVFEFPNFRK GTEIVANAIA DSEAFSIAGG GDTLAAIDLF GIADKISYIS 370 380 TGGGAFLEFV EGKVLPAVAM LEERAKK

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed: 17293413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000468 Genomic DNA. Translation: ABJ02350.1.
RefSeq	YP_854139.1. NC_008563.1.
3D structure databases
ProteinModelPortal	A1AFB0.
SMR	A1AFB0. Positions 2-387.
ModBase	Search...
Protein-protein interaction databases
STRING	A1AFB0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000036216; EBESCP00000034733; EBESCG00000035266.
GeneID	4495157.
GenomeReviews	Gene locus Ecok1_28560 in contig CP000468_GR.
KEGG	ecv:APECO1_3607.
PATRIC	18218011. VBIEscCol127180_3245.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0126.
GeneTree	EBGT00050000011026.
HOGENOM	HBG453500.
OMA	DCIGDEV.
ProtClustDB	PRK00073.
Enzyme and pathway databases
BioCyc	ECOL405955:APECO1_3607-MONOMER.
Family and domain databases
HAMAP	MF_00145. Phosphoglyc_kinase. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
KO	K00927.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_ECOK1

Accession

Primary (citable) accession number: A1AFB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program