A1AEU1 (ISPD_ECOK1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	Ecok1_26870
ORF Names:	APECO1_3776

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP-Rule MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP-Rule MF_00108
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP-Rule MF_00108		PRO_1000022922
Sites
	Site	20	1	Transition state stabilizer By similarity
	Site	27	1	Transition state stabilizer By similarity
	Site	157	1	Positions MEP for the nucleophilic attack By similarity
	Site	213	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1AEU1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: E2065C781E122202	FASTA	236	25,779
	10 20 30 40 50 60 MATTHLDVCA VVPAAGFGRR MQTECPKQYL SIGNQTILEH SVHALLAHPR VKRVVIAISP 70 80 90 100 110 120 GDSRFAQLPL ANHPRITVVD GGEERADSVL AGLKAAGDAQ WVLVHDAARP CLHQDDLARL 130 140 150 160 170 180 LALSETSRTG GILAAPVRDT MKRAEPGKNA IAHTVDRNGL WHALTPQFFP RELLHDCLTR 190 200 210 220 230 ALNEGATITD EASALEYCGF HPQLVEGRAD NIKVTRPEDL ALAEFYLTRT IHQENT

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000468 Genomic DNA. Translation: ABJ02181.1.
RefSeq	YP_853970.1. NC_008563.1.
3D structure databases
ProteinModelPortal	A1AEU1.
SMR	A1AEU1. Positions 5-229.
ModBase	Search...
Protein-protein interaction databases
STRING	405955.APECO1_3776.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ02181; ABJ02181; APECO1_3776.
GeneID	4494590.
KEGG	ecv:APECO1_3776.
PATRIC	18217635. VBIEscCol127180_3061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HOG000218564.
KO	K00991.
OMA	IIGQPKD.
ProtClustDB	PRK00155.
Enzyme and pathway databases
UniPathway	UPA00056; UER00093.
Family and domain databases
HAMAP	MF_00108. IspD.
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	A1AEU1.

Entry information

Entry name

ISPD_ECOK1

Accession

Primary (citable) accession number: A1AEU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program