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Protein

tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC

Gene

mnmC

Organism
Escherichia coli O1:K1 / APEC
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm5s2U34 to nm5s2U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm5s2U34, to form mnm5s2U34.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.UniRule annotation

Cofactori

FADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCUniRule annotation
Short name:
tRNA mnm(5)s(2)U biosynthesis bifunctional proteinUniRule annotation
Including the following 2 domains:
tRNA (mnm(5)s(2)U34)-methyltransferaseUniRule annotation (EC:2.1.1.61UniRule annotation)
FAD-dependent cmnm(5)s(2)U34 oxidoreductaseUniRule annotation (EC:1.5.-.-UniRule annotation)
Gene namesi
Name:mnmCUniRule annotation
Ordered Locus Names:Ecok1_22190
ORF Names:APECO1_4240
OrganismiEscherichia coli O1:K1 / APEC
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008216 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000649941 – 668tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCAdd BLAST668

Structurei

3D structure databases

ProteinModelPortaliA1ADH3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 245tRNA (mnm(5)s(2)U34)-methyltransferaseAdd BLAST245
Regioni270 – 668FAD-dependent cmnm(5)s(2)U34 oxidoreductaseAdd BLAST399

Sequence similaritiesi

In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the DAO family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218142.
KOiK15461.
OMAiKSVLCYD.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.

Sequencei

Sequence statusi: Complete.

A1ADH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHYSIQPAN LEFNAEGTPV SRDFDDVYFS NDNGLEETRY VFLGGNHLEA
60 70 80 90 100
RFPEHPHPLF VVAESGFGTG LNFLTLWQAF DQFREAHPQA QLQRLHFISF
110 120 130 140 150
EKFPLTRADL ALAHQHWPEL APWAEQLQAQ WPLPLPGCHR LLLDEGRITL
160 170 180 190 200
DLWFGDINEL TSQLDDSLNQ KVDAWFLDGF APAKNPDMWT QNLFNAMARL
210 220 230 240 250
ARPGSTLATF TSAGFVRRGL QEAGFTMQKR KGFGRKREML CGVMEQTLPL
260 270 280 290 300
PCSTPWFNRT GSNKQEAAII GGGIASALLS LALLRRGWQV TLYCADEAPA
310 320 330 340 350
LGASGNRQGA LYPLLSKHDE ALNRFFSNAF TFARRFYDLL PVKFDHDWCG
360 370 380 390 400
VTQLGWDEKS QHKIAQMLSM DLPAELAVAV EANAVEQITG VATNCSGITY
410 420 430 440 450
PQGGWLCPAE LTRNVLKLAQ QQGLQIHYQY QLQDLSRKDD GWLLNFAGDQ
460 470 480 490 500
QATHSVVVLA NGHQISRFSQ TSSLPVYSVA GQVSHIPTTP ELAELKQVLC
510 520 530 540 550
YDGYLTPQNT ANQHHCIGAS YHRGSEETAY SDEDQQQNRQ RLIDCFPHAQ
560 570 580 590 600
WAKEVDVSGK EARCGVRCAT RDHLPMVGNV PDYDATLVEY ASLAEKKDEA
610 620 630 640 650
VSAPVYDDLF MFAALGSRGL CSAPLCAEIL AAQMSEEPIP MDASTLAALN
660
PNRLWVRKLL KGKAVKAG
Length:668
Mass (Da):74,368
Last modified:January 23, 2007 - v1
Checksum:i774729A085048EE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ01713.1.
RefSeqiWP_000683753.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABJ01713; ABJ01713; APECO1_4240.
KEGGiecv:APECO1_4240.
PATRICi18216575. VBIEscCol127180_2549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ01713.1.
RefSeqiWP_000683753.1. NC_008563.1.

3D structure databases

ProteinModelPortaliA1ADH3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ01713; ABJ01713; APECO1_4240.
KEGGiecv:APECO1_4240.
PATRICi18216575. VBIEscCol127180_2549.

Phylogenomic databases

HOGENOMiHOG000218142.
KOiK15461.
OMAiKSVLCYD.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNMC_ECOK1
AccessioniPrimary (citable) accession number: A1ADH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.