A1ACW2 (GATY_ECOK1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-tagatose-1,6-bisphosphate aldolase subunit GatY
Short name=TBPA
Short name=TagBP aldolase
EC=4.1.2.40

Alternative name(s):
D-tagatose-bisphosphate aldolase class II
Tagatose-bisphosphate aldolase

Gene names

Name:	gatY
Ordered Locus Names:	Ecok1_20080
ORF Names:	APECO1_4449

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the tagatose-1,6-bisphosphate aldolase GatYZ, which catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6-bisphosphate (TBP). Requires GatZ subunit for full activity and stability. Is involved in the catabolism of galactitol By similarity. HAMAP-Rule MF_01294
Catalytic activity	D-tagatose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. HAMAP-Rule MF_01294
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01294
Pathway	Carbohydrate metabolism; D-tagatose 6-phosphate degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6-phosphate: step 2/2. HAMAP-Rule MF_01294
Subunit structure	Forms a complex with GatZ By similarity. HAMAP-Rule MF_01294
Sequence similarities	Belongs to the class II fructose-bisphosphate aldolase family. TagBP aldolase GatY subfamily.

Ontologies

Keywords
Biological process	Galactitol metabolism
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-tagatose 6-phosphate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway galactitol catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	tagatose-bisphosphate aldolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	D-tagatose-1,6-bisphosphate aldolase subunit GatY HAMAP-Rule MF_01294		PRO_0000355336
Regions
	Region	209 – 211	3	Dihydroxyacetone phosphate binding By similarity
	Region	230 – 233	4	Dihydroxyacetone phosphate binding By similarity
Sites
	Active site	82	1	Proton donor By similarity
	Metal binding	83	1	Zinc; catalytic By similarity
	Metal binding	180	1	Zinc; catalytic By similarity
	Metal binding	208	1	Zinc; catalytic By similarity
	Binding site	181	1	Dihydroxyacetone phosphate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1ACW2 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 9C920999A2708E8C	FASTA	284	30,937
	10 20 30 40 50 60 MYVVSTKQML NNARRGGYAV PAFNIHNLET MQVVVETAAS MHAPVIIAGT PGTFTHAGTE 70 80 90 100 110 120 NLMALVSAMA KQYHHPLAIH LDHHTKFDDI AQKVRSGVRS VMIDASHLPF AQNISRVKEV 130 140 150 160 170 180 VDFCHRFDVS VEAELGQLGG QEDDVQVNEA DAFYTNPVQA REFAEATGID SLAVAIGTAH 190 200 210 220 230 240 GMYASAPALD FSRLENIRQW VNLPLVLHGA SGLSTKDIQQ TIKLGICKIN VATELKNAFS 250 260 270 280 QALKNYLTEY PEATDPRDYL QSAKSAMRDV VSKVIADCGC EGRA

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000468 Genomic DNA. Translation: ABJ01502.1.
RefSeq	YP_853216.1. NC_008563.1.
3D structure databases
ProteinModelPortal	A1ACW2.
SMR	A1ACW2. Positions 2-284.
ModBase	Search...
Protein-protein interaction databases
STRING	405955.APECO1_4449.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ01502; ABJ01502; APECO1_4449.
GeneID	4494506.
KEGG	ecv:APECO1_4449.
PATRIC	18216091. VBIEscCol127180_2309.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0191.
HOGENOM	HOG000227793.
KO	K08302.
OMA	KMYVVST.
ProtClustDB	PRK09195.
Enzyme and pathway databases
UniPathway	UPA00704; UER00716.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01294. TagBP_aldolase_GatY.
InterPro	IPR013785. Aldolase_TIM. IPR000771. Ketose_bisP_aldolase_II. IPR011288. TagBP_ald_KbaY/GatY. IPR023955. TagBP_aldolase_GatY. [Graphical view]
Pfam	PF01116. F_bP_aldolase. 1 hit. [Graphical view]
PIRSF	PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMs	TIGR00167. cbbA. 1 hit. TIGR01858. tag_bisphos_ald. 1 hit.
PROSITE	PS00602. ALDOLASE_CLASS_II_1. 1 hit. PS00806. ALDOLASE_CLASS_II_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATY_ECOK1

Accession

Primary (citable) accession number: A1ACW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program