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Protein

Protein-methionine-sulfoxide reductase catalytic subunit MsrP

Gene

msrP

Organism
Escherichia coli O1:K1 / APEC
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons. Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine generated by the host defense mechanisms. MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation, including the primary periplasmic chaperone SurA and the lipoprotein Pal. The catalytic subunit MsrP is non-stereospecific, being able to reduce both (R-) and (S-) diastereoisomers of methionine sulfoxide.UniRule annotation

Catalytic activityi

[Protein]-L-methionine + a quinone + H2O = [protein]-L-methionine (S)-S-oxide + a quinol.UniRule annotation
[Protein]-L-methionine + a quinone + H2O = [protein]-L-methionine (R)-S-oxide + a quinol.UniRule annotation

Cofactori

Mo-molybdopterinUniRule annotationNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881MolybdopterinUniRule annotation
Metal bindingi146 – 1461MolybdenumUniRule annotation
Binding sitei181 – 1811MolybdopterinUniRule annotation
Binding sitei233 – 2331MolybdopterinUniRule annotation
Binding sitei238 – 2381MolybdopterinUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-methionine-sulfoxide reductase catalytic subunit MsrPUniRule annotation (EC:1.8.5.-UniRule annotation)
Gene namesi
Name:msrPUniRule annotation
Ordered Locus Names:Ecok1_18120
ORF Names:APECO1_1006
OrganismiEscherichia coli O1:K1 / APEC
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008216 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

  • Note: Is attached to the inner membrane when interacting with the MsrQ subunit.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4444Tat-type signalUniRule annotationAdd
BLAST
Chaini45 – 334290Protein-methionine-sulfoxide reductase catalytic subunit MsrPUniRule annotationPRO_1000066157Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Heterodimer of a catalytic subunit (MsrP) and a heme-binding subunit (MsrQ).UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA1ACB6.
SMRiA1ACB6. Positions 62-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 922Molybdopterin bindingUniRule annotation
Regioni249 – 2513Molybdopterin bindingUniRule annotation

Sequence similaritiesi

Belongs to the MsrP family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000255004.
KOiK07147.
OMAiDWPYVEG.

Family and domain databases

Gene3Di3.90.420.10. 1 hit.
HAMAPiMF_01206. MsrP. 1 hit.
InterProiIPR022867. MsrP.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF00174. Oxidored_molyb. 1 hit.
[Graphical view]
SUPFAMiSSF56524. SSF56524. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1ACB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNQFLKES DVTAESVFFM KRRQVLKALG ISAAALSLPH AAHADLLSWF
60 70 80 90 100
KGNDRPPAPA GKALEFSKPA AWQNNLPLTP ADKVSGYNNF YEFGLDKADP
110 120 130 140 150
AANAGSLKTD PWTLKISGEV AKPLTLDHDD LTRRFPLEER IYRMRCVEAW
160 170 180 190 200
SMVVPWIGFP LHKLLALAEP TSNAKYVAFE TIYAPEQMPG QQDRFIGGGL
210 220 230 240 250
KYPYVEGLRL DEAMHPLTLM TVGVYGKALP PQNGAPVRLI VPWKYGFKGI
260 270 280 290 300
KSIVSIKLTR ERPPTTWNLA APDEYGFYAN VNPHVDHPRW SQATERFIGS
310 320 330
GGILNVQRQP TLLFNGYAEQ VASLYRGLDL RENF
Length:334
Mass (Da):37,370
Last modified:January 23, 2007 - v1
Checksum:iA6A0FF0A2E349006
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ01306.1.
RefSeqiWP_000740082.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABJ01306; ABJ01306; APECO1_1006.
KEGGiecv:APECO1_1006.
PATRICi18215585. VBIEscCol127180_2065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ01306.1.
RefSeqiWP_000740082.1. NC_008563.1.

3D structure databases

ProteinModelPortaliA1ACB6.
SMRiA1ACB6. Positions 62-330.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ01306; ABJ01306; APECO1_1006.
KEGGiecv:APECO1_1006.
PATRICi18215585. VBIEscCol127180_2065.

Phylogenomic databases

HOGENOMiHOG000255004.
KOiK07147.
OMAiDWPYVEG.

Family and domain databases

Gene3Di3.90.420.10. 1 hit.
HAMAPiMF_01206. MsrP. 1 hit.
InterProiIPR022867. MsrP.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF00174. Oxidored_molyb. 1 hit.
[Graphical view]
SUPFAMiSSF56524. SSF56524. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSRP_ECOK1
AccessioniPrimary (citable) accession number: A1ACB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.