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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Escherichia coli O1:K1 / APEC
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei48 – 481UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 538ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:Ecok1_16170
ORF Names:APECO1_809
OrganismiEscherichia coli O1:K1 / APEC
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008216 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275NH(3)-dependent NAD(+) synthetasePRO_1000077551Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA1ABS1.
SMRiA1ABS1. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000238070.
KOiK01916.
OMAiAKIIEGW.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

A1ABS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQQQIIKV LGAKPQINAE EEIRRSIDFL KSYLQTYPFI KSLVLGISGG
60 70 80 90 100
QDSTLAGKLC QMAINELRQE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF
110 120 130 140 150
IQPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA
160 170 180 190 200
GMTSGVVVGT DHAAEAITGF FTKYGDGGTD INPLYRLNKR QGKQLLAALG
210 220 230 240 250
CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG KNVPEQVART
260 270
IENWYLKTEH KRRPPITVFD DFWKK
Length:275
Mass (Da):30,681
Last modified:January 23, 2007 - v1
Checksum:i268EB153AF4579D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ01111.1.
RefSeqiWP_000175056.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABJ01111; ABJ01111; APECO1_809.
KEGGiecv:APECO1_809.
PATRICi18215106. VBIEscCol127180_1829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ01111.1.
RefSeqiWP_000175056.1. NC_008563.1.

3D structure databases

ProteinModelPortaliA1ABS1.
SMRiA1ABS1. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ01111; ABJ01111; APECO1_809.
KEGGiecv:APECO1_809.
PATRICi18215106. VBIEscCol127180_1829.

Phylogenomic databases

HOGENOMiHOG000238070.
KOiK01916.
OMAiAKIIEGW.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_ECOK1
AccessioniPrimary (citable) accession number: A1ABS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.