A1AB75 (MAO1_ECOK1) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 46.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: NAD-dependent malic enzyme Short name=NAD-ME EC=1.1.1.38 | ||||||
| Gene names |
| ||||||
| Organism | Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 405955 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 565 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619 |
| Cofactor | Divalent metal cations. Prefers magnesium or manganese By similarity. |
| Subunit structure | Homotetramer By similarity. HAMAP MF_01619 |
| Sequence similarities | Belongs to the malic enzymes family. |
| Sequence caution | The sequence ABJ00915.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | malate metabolic process Inferred from electronic annotation. Source: InterPro |
| Molecular function | NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activityInferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 565 | 565 | NAD-dependent malic enzyme HAMAP MF_01619 | PRO_0000323538 | |||||
Sites | |||||||||
| Active site | 104 | 1 | Proton donor By similarity | ||||||
| Active site | 175 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 246 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 247 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 270 | 1 | Divalent metal cation By similarity | ||||||
| Binding site | 157 | 1 | NAD By similarity | ||||||
| Binding site | 270 | 1 | NAD By similarity | ||||||
| Binding site | 418 | 1 | NAD By similarity | ||||||
| Site | 270 | 1 | Important for activity By similarity | ||||||
Sequences
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References
| [1] | "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed: 17293413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CP000468 Genomic DNA. Translation: ABJ00915.1. Different initiation. |
| RefSeq | YP_852629.2. NC_008563.1. |
3D structure databases | |
| ProteinModelPortal | A1AB75. |
| SMR | A1AB75. Positions 15-564. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | A1AB75. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000038204; EBESCP00000036721; EBESCG00000037254. |
| GeneID | 4494401. |
| GenomeReviews | Gene locus Ecok1_14210 in contig CP000468_GR. |
| KEGG | ecv:APECO1_615. |
| PATRIC | 18214646. VBIEscCol127180_1602. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG0281. |
| GeneTree | EBGT00050000008964. |
| HOGENOM | HBG289821. |
| ProtClustDB | PRK13529. |
Enzyme and pathway databases | |
| BioCyc | ECOL405955:APECO1_615-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01619. NAD_malic_enz. [Tree] |
| InterPro | IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. IPR023667. NAD_malic_enz_proteobac. [Graphical view] |
| Gene3D | G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K00027. |
| Pfam | PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view] |
| PIRSF | PIRSF000106. ME. 1 hit. |
| PRINTS | PR00072. MALOXRDTASE. |
| SMART | SM00919. Malic_M. 1 hit. [Graphical view] |
| PROSITE | PS00331. MALIC_ENZYMES. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MAO1_ECOK1 | ||||||||
| Accession | Primary (citable) accession number: A1AB75 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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