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A1AA51

- RUSA_ECOK1

UniProt

A1AA51 - RUSA_ECOK1

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Protein

Crossover junction endodeoxyribonuclease RusA

Gene

rusA

Organism
Escherichia coli O1:K1 / APEC
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC By similarity.By similarity

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Binds 1 magnesium ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701MagnesiumBy similarity
Metal bindingi72 – 721MagnesiumBy similarity
Metal bindingi91 – 911MagnesiumBy similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase
Gene namesi
Name:rusA
Ordered Locus Names:Ecok1_10470
ORF Names:APECO1_239
OrganismiEscherichia coli O1:K1 / APEC
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000008216: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120Crossover junction endodeoxyribonuclease RusAPRO_0000324836Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi405955.APECO1_239.

Structurei

3D structure databases

ProteinModelPortaliA1AA51.
SMRiA1AA51. Positions 1-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 164DNA bindingBy similarity
Regioni66 – 738DNA bindingBy similarity

Sequence similaritiesi

Belongs to the RusA family.Curated

Phylogenomic databases

eggNOGiCOG4570.
HOGENOMiHOG000119986.
KOiK01160.
OMAiRGIHYIS.
OrthoDBiEOG66HVH9.

Family and domain databases

Gene3Di3.30.1330.70. 1 hit.
InterProiIPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamiPF05866. RusA. 1 hit.
[Graphical view]
PIRSFiPIRSF001007. RusA. 1 hit.
SUPFAMiSSF103084. SSF103084. 1 hit.

Sequencei

Sequence statusi: Complete.

A1AA51-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG
60 70 80 90 100
LAMPVKIRIE CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV
110 120
KMPVTKGGRL ELTITEMGNE
Length:120
Mass (Da):13,846
Last modified:January 23, 2007 - v1
Checksum:i38401669E8819EA6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000468 Genomic DNA. Translation: ABJ00541.1.
RefSeqiYP_852255.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABJ00541; ABJ00541; APECO1_239.
GeneIDi4491808.
KEGGiecv:APECO1_239.
PATRICi18213748. VBIEscCol127180_1160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000468 Genomic DNA. Translation: ABJ00541.1 .
RefSeqi YP_852255.1. NC_008563.1.

3D structure databases

ProteinModelPortali A1AA51.
SMRi A1AA51. Positions 1-118.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405955.APECO1_239.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ00541 ; ABJ00541 ; APECO1_239 .
GeneIDi 4491808.
KEGGi ecv:APECO1_239.
PATRICi 18213748. VBIEscCol127180_1160.

Phylogenomic databases

eggNOGi COG4570.
HOGENOMi HOG000119986.
KOi K01160.
OMAi RGIHYIS.
OrthoDBi EOG66HVH9.

Family and domain databases

Gene3Di 3.30.1330.70. 1 hit.
InterProi IPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view ]
Pfami PF05866. RusA. 1 hit.
[Graphical view ]
PIRSFi PIRSF001007. RusA. 1 hit.
SUPFAMi SSF103084. SSF103084. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes."
    Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.
    J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRUSA_ECOK1
AccessioniPrimary (citable) accession number: A1AA51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3