A1A9S3 (EFEB_ECOK1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deferrochelatase/peroxidase EfeB
EC=1.11.1.-

Gene names

Name:	efeB
Ordered Locus Names:	Ecok1_09190

Organism

Escherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]

Taxonomic identifier

405955 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the recovery of exogenous heme iron. Extracts iron from heme while preserving the tetrapyrrol ring intact By similarity.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group non-covalently per subunit By similarity.
Subunit structure	Homodimer. Part of a ferrous iron transporter composed of EfeU, EfeO and EfeB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the DyP-type peroxidase family. EfeB subfamily.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Tat-type signal Potential
	Chain	36 – 423	388	Deferrochelatase/peroxidase EfeB		PRO_0000278545
Regions
	Region	236 – 238	3	Heme binding By similarity
	Region	334 – 336	3	Heme binding By similarity
Sites
	Metal binding	329	1	Iron (heme proximal ligand); via tele nitrogen By similarity
	Binding site	347	1	Heme By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1A9S3 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 9EB660519206CD2E	FASTA	423	46,654
	10 20 30 40 50 60 MQYEDENGVN EPSRRRLLKG IGALALAGSC PVAHAQKTQS APGTLSPDAR NEKQPFYGEH 70 80 90 100 110 120 QAGILTPQQA AMMLVAFDVL ASDKADLERL FRLLTQRFAF LTQGGAAPET PNPRLPPLDS 130 140 150 160 170 180 GILGGYIAPD NLTITLSVGH SLFDERFGLA PQMPKKLQKM TRFPNDSLDA ALCHGDVLLQ 190 200 210 220 230 240 ICANTQDTVI HALRDIIKHT PDLLSVRWKR EGFISDHAAR SKGKETPINL LGFKDGTANP 250 260 270 280 290 300 DSQNDKLMQK VVWVTADQQE PAWTIGGSYQ AVRLIQFRVE FWDRTPLKEQ QTIFGRDKQT 310 320 330 340 350 360 GAPLGMLHEH DVPDYASDPE GKVIALDSHI RLANPRTAES ESSLMLRRGY SYSLGVTNSG 370 380 390 400 410 420 QLDMGLLFVC YQHDLEKGFL TVQKRLNGEA LEEYVKPIGG GYFFALSGVK DANDYLGSAL LRV

References

[1]

"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes." Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K. J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000468 Genomic DNA. Translation: ABJ00413.1.
RefSeq	YP_852127.1. NC_008563.1.
3D structure databases
ProteinModelPortal	A1A9S3.
ModBase	Search...
Protein-protein interaction databases
STRING	405955.APECO1_110.
Protein family/group databases
PeroxiBase	5878. EcoDyPrx01_APECO1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ00413; ABJ00413; APECO1_110.
GeneID	4495215.
KEGG	ecv:APECO1_110.
PATRIC	18213474. VBIEscCol127180_1024.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2837.
KO	K16301.
OMA	VERWDRT.
ProtClustDB	CLSK879930.
Family and domain databases
InterPro	IPR011008. Dimeric_a/b-barrel. IPR006314. Dyp_peroxidase. IPR006313. Tat_enzyme. IPR006311. TAT_signal. [Graphical view]
Pfam	PF04261. Dyp_perox. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
TIGRFAMs	TIGR01413. Dyp_perox_fam. 1 hit. TIGR01412. tat_substr_1. 1 hit.
PROSITE	PS51404. DYP_PEROXIDASE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFEB_ECOK1

Accession

Primary (citable) accession number: A1A9S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program