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Protein

Deferrochelatase/peroxidase EfeB

Gene

efeB

Organism
Escherichia coli O1:K1 / APEC
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the recovery of exogenous heme iron. Extracts iron from heme while preserving the tetrapyrrol ring intact (By similarity).By similarity

Cofactori

heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group non-covalently per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi329 – 3291Iron (heme proximal ligand); via tele nitrogenBy similarity
Binding sitei347 – 3471HemeBy similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  1. ferrous iron transport Source: InterPro
  2. iron assimilation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Heme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei5878. EcoDyPrx01_APECO1.

Names & Taxonomyi

Protein namesi
Recommended name:
Deferrochelatase/peroxidase EfeB (EC:1.11.1.-)
Gene namesi
Name:efeB
Ordered Locus Names:Ecok1_09190
OrganismiEscherichia coli O1:K1 / APEC
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000008216: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini36 – 423388Deferrochelatase/peroxidase EfeBPRO_0000278545Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Homodimer. Part of a ferrous iron transporter composed of EfeU, EfeO and EfeB (By similarity).By similarity

Protein-protein interaction databases

STRINGi405955.APECO1_110.

Structurei

3D structure databases

ProteinModelPortaliA1A9S3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni236 – 2383Heme bindingBy similarity
Regioni334 – 3363Heme bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2837.
KOiK16301.
OMAiLPPMDSG.
OrthoDBiEOG6F55G9.

Family and domain databases

InterProiIPR011008. Dimeric_a/b-barrel.
IPR006314. Dyp_peroxidase.
IPR006313. EfeB.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04261. Dyp_perox. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
TIGRFAMsiTIGR01413. Dyp_perox_fam. 1 hit.
TIGR01412. tat_substr_1. 1 hit.
PROSITEiPS51404. DYP_PEROXIDASE. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1A9S3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYEDENGVN EPSRRRLLKG IGALALAGSC PVAHAQKTQS APGTLSPDAR
60 70 80 90 100
NEKQPFYGEH QAGILTPQQA AMMLVAFDVL ASDKADLERL FRLLTQRFAF
110 120 130 140 150
LTQGGAAPET PNPRLPPLDS GILGGYIAPD NLTITLSVGH SLFDERFGLA
160 170 180 190 200
PQMPKKLQKM TRFPNDSLDA ALCHGDVLLQ ICANTQDTVI HALRDIIKHT
210 220 230 240 250
PDLLSVRWKR EGFISDHAAR SKGKETPINL LGFKDGTANP DSQNDKLMQK
260 270 280 290 300
VVWVTADQQE PAWTIGGSYQ AVRLIQFRVE FWDRTPLKEQ QTIFGRDKQT
310 320 330 340 350
GAPLGMLHEH DVPDYASDPE GKVIALDSHI RLANPRTAES ESSLMLRRGY
360 370 380 390 400
SYSLGVTNSG QLDMGLLFVC YQHDLEKGFL TVQKRLNGEA LEEYVKPIGG
410 420
GYFFALSGVK DANDYLGSAL LRV
Length:423
Mass (Da):46,654
Last modified:January 23, 2007 - v1
Checksum:i9EB660519206CD2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ00413.1.
RefSeqiYP_852127.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABJ00413; ABJ00413; APECO1_110.
GeneIDi4495215.
KEGGiecv:APECO1_110.
PATRICi18213474. VBIEscCol127180_1024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ00413.1.
RefSeqiYP_852127.1. NC_008563.1.

3D structure databases

ProteinModelPortaliA1A9S3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405955.APECO1_110.

Protein family/group databases

PeroxiBasei5878. EcoDyPrx01_APECO1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ00413; ABJ00413; APECO1_110.
GeneIDi4495215.
KEGGiecv:APECO1_110.
PATRICi18213474. VBIEscCol127180_1024.

Phylogenomic databases

eggNOGiCOG2837.
KOiK16301.
OMAiLPPMDSG.
OrthoDBiEOG6F55G9.

Family and domain databases

InterProiIPR011008. Dimeric_a/b-barrel.
IPR006314. Dyp_peroxidase.
IPR006313. EfeB.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04261. Dyp_perox. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
TIGRFAMsiTIGR01413. Dyp_perox_fam. 1 hit.
TIGR01412. tat_substr_1. 1 hit.
PROSITEiPS51404. DYP_PEROXIDASE. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes."
    Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.
    J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiEFEB_ECOK1
AccessioniPrimary (citable) accession number: A1A9S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.