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Protein

Ribosomal RNA large subunit methyltransferase F

Gene

rlmF

Organism
Escherichia coli O1:K1 / APEC
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the adenine in position 1618 of 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase FUniRule annotation (EC:2.1.1.181UniRule annotation)
Alternative name(s):
23S rRNA mA1618 methyltransferaseUniRule annotation
rRNA adenine N-6-methyltransferaseUniRule annotation
Gene namesi
Name:rlmFUniRule annotation
Ordered Locus Names:Ecok1_06930
ORF Names:APECO1_1284
OrganismiEscherichia coli O1:K1 / APEC
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000008216 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Ribosomal RNA large subunit methyltransferase FPRO_0000349912Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA1A947.
SMRiA1A947. Positions 44-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily. METTL16/RlmF family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218605.
KOiK06970.
OrthoDBiEOG6742SX.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01848. 23SrRNA_methyltr_F.
InterProiIPR016909. rRNA_lsu_MeTfrase_F.
IPR010286. rRNA_lsu_MeTfrase_F-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF029038. Mtase_YbiN_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

A1A947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAQKPGLHP RNRHHSRYDL ATLCQVNPEL RQFLTLTPAG EQSVDFANPL
60 70 80 90 100
AVKALNKALL AHFYAVANWD IPDGFLCPPV PGRADYIHHL ADLLAEASGT
110 120 130 140 150
IPANASILDI GVGANCIYPL IGVHEYGWRF TGSETSSQAL SSAQAIISAN
160 170 180 190 200
PGLNRAIRLR RQKESGAIFN GIIHKNEQYD ATLCNPPFHD SAAAARAGSE
210 220 230 240 250
RKRRNLGLNK DDALNFGGQQ QELWCEGGEV AFIKKMIEES KGFAKQVMWF
260 270 280 290 300
TSLVSRGENL PPLYRALTDV GAVKVVKKEM AQGQKQSRFI AWTFMNDEQR

RRFVNRQR
Length:308
Mass (Da):34,180
Last modified:September 2, 2008 - v2
Checksum:i10D0EA4C2F3E23D3
GO

Sequence cautioni

The sequence ABJ00187.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ00187.1. Different initiation.
RefSeqiWP_001275941.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABJ00187; ABJ00187; APECO1_1284.
KEGGiecv:APECO1_1284.
PATRICi18212954. VBIEscCol127180_0766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABJ00187.1. Different initiation.
RefSeqiWP_001275941.1. NC_008563.1.

3D structure databases

ProteinModelPortaliA1A947.
SMRiA1A947. Positions 44-296.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ00187; ABJ00187; APECO1_1284.
KEGGiecv:APECO1_1284.
PATRICi18212954. VBIEscCol127180_0766.

Phylogenomic databases

HOGENOMiHOG000218605.
KOiK06970.
OrthoDBiEOG6742SX.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01848. 23SrRNA_methyltr_F.
InterProiIPR016909. rRNA_lsu_MeTfrase_F.
IPR010286. rRNA_lsu_MeTfrase_F-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF029038. Mtase_YbiN_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes."
    Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.
    J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRLMF_ECOK1
AccessioniPrimary (citable) accession number: A1A947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: December 9, 2015
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.