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Reviewed, UniProtKB/Swiss-Prot A1A876 (TGT_ECOK1)

Last modified June 16, 2009. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme
Gene names
Name: tgt
Ordered Locus Names: Ecok1_03720
ORF Names: APECO1_1604
OrganismEscherichia coli O1:K1 / APEC [Complete proteome] [HAMAP]
Taxonomic identifier405955 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.

Catalytic activity

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168

[tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

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Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular functionqueuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 375375Queuine tRNA-ribosyltransferase HAMAP MF_00168
PRO_1000016787

Sites

Active site891Nucleophile By similarity
Metal binding3021Zinc By similarity
Metal binding3041Zinc By similarity
Metal binding3071Zinc By similarity
Metal binding3331Zinc By similarity
Binding site901Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
A1A876-1 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 40271CA08D8A8820

FASTA37542,594
        10         20         30         40         50         60 
MKFELDTTDG RARRGRLVFD RGVVETPCFM PVGTYGTVKG MTPEEVEATG AQIILGNTFH 

        70         80         90        100        110        120 
LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD IRKITEQGVH FRNPINGDPI 

       130        140        150        160        170        180 
FLDPEKSMEI QYDLGSDIVM IFDECTPYPA DWDYAKRSME MSLRWAKRSR ERFDSLGNKN 

       190        200        210        220        230        240 
ALFGIIQGSV YEDLRDISVK GLVDIGFDGY AVGGLAVGEP KADMHRILEH VCPQIPADKP 

       250        260        270        280        290        300 
RYLMGVGKPE DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK YKSDTGPLDP 

       310        320        330        340        350        360 
ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE EGKLESFVTD 

       370 
FYQRQGREVP PLNVD

« Hide

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References

[1]"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes."
Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.
J. Bacteriol. 189:3228-3236(2007) [PubMed: 17293413] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000468 Genomic DNA. Translation: ABI99865.1.
RefSeqYP_851580.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID4492479.
GenomeReviewsGene locus Ecok1_03720 in contig CP000468_GR.
KEGGecv:APECO1_1604.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAA1A876. VLNSDIV.

Family and domain databases

HAMAPMF_00168.
[Tree]
InterProIPR004803. QtRNA_ribo_trans.
IPR002616. tRNA_ribo_trans.
[Graphical view]
Gene3DG3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHERPTHR11962. tRNA_ribo_trans. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameTGT_ECOK1
AccessionPrimary (citable) accession number: A1A876
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents