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Protein

Ribonuclease H

Gene

rnhA

Organism
Escherichia coli O1:K1 / APEC
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotationSAAS annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotationNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Magnesium 1UniRule annotation
Metal bindingi47 – 471Magnesium 2UniRule annotation
Metal bindingi85 – 851Magnesium 1UniRule annotation
Metal bindingi107 – 1071Magnesium 1UniRule annotation
Metal bindingi171 – 1711Magnesium 2UniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. nucleic acid binding Source: InterPro
  3. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseUniRule annotationSAAS annotation, Hydrolase, Nuclease

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HUniRule annotationSAAS annotation (EC:3.1.26.4UniRule annotationSAAS annotation)
Short name:
RNase HUniRule annotation
Gene namesi
Name:rnhAUniRule annotationImported
ORF Names:APECO1_1776Imported
OrganismiEscherichia coli O1:K1 / APECImported
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000008216 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi405955.APECO1_1776.

Structurei

3D structure databases

ProteinModelPortaliA1A7Q5.
SMRiA1A7Q5. Positions 38-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 179142RNase HUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RNase H family.UniRule annotation
Contains 1 RNase H domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0328.
HOGENOMiHOG000040465.
KOiK03469.
OMAiITSWIHN.
OrthoDBiEOG696BTR.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1A7Q5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSVSRTIWR VIAVLIAVIY VRLIVLQFDS ITGSLPEMLK QVEIFTDGSC
60 70 80 90 100
LGNPGPGGYG AILRYRGREK TFSAGYTRTT NNRMELMAAI VALEALKEHC
110 120 130 140 150
EVILSTDSQY VRQGITQWIH NWKKRGWKTA DKKPVKNVDL WQRLDAALGQ
160 170 180 190
HQIKWEWVKG HAGHPENERC DELARAAAMN PTLEDTGYQV EV
Length:192
Mass (Da):21,738
Last modified:January 23, 2007 - v1
Checksum:i7C59C9837F87BE98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABI99694.1.
RefSeqiYP_851409.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABI99694; ABI99694; APECO1_1776.
KEGGiecv:APECO1_1776.
PATRICi18211827. VBIEscCol127180_0221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000468 Genomic DNA. Translation: ABI99694.1.
RefSeqiYP_851409.1. NC_008563.1.

3D structure databases

ProteinModelPortaliA1A7Q5.
SMRiA1A7Q5. Positions 38-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405955.APECO1_1776.

Chemistry

BindingDBiA1A7Q5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI99694; ABI99694; APECO1_1776.
KEGGiecv:APECO1_1776.
PATRICi18211827. VBIEscCol127180_0221.

Phylogenomic databases

eggNOGiCOG0328.
HOGENOMiHOG000040465.
KOiK03469.
OMAiITSWIHN.
OrthoDBiEOG696BTR.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes."
    Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.
    J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APEC O1Imported.

Entry informationi

Entry nameiA1A7Q5_ECOK1
AccessioniPrimary (citable) accession number: A1A7Q5
Entry historyi
Integrated into UniProtKB/TrEMBL: January 23, 2007
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.