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A1A7L2

- A1A7L2_ECOK1

UniProt

A1A7L2 - A1A7L2_ECOK1

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Protein
Methionine aminopeptidase
Gene
map, APECO1_1819
Organism
Escherichia coli O1:K1 / APEC
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins By similarity.UniRule annotation
Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed By similarity.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 cobalt ions per subunit By similarity.UniRule annotation
Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791Substrate By similarityUniRule annotation
Metal bindingi97 – 971Divalent metal cation 1 By similarityUniRule annotation
Metal bindingi108 – 1081Divalent metal cation 1 By similarityUniRule annotation
Metal bindingi108 – 1081Divalent metal cation 2; catalytic By similarityUniRule annotation
Metal bindingi171 – 1711Divalent metal cation 2; catalytic; via tele nitrogen By similarityUniRule annotation
Binding sitei178 – 1781Substrate By similarityUniRule annotation
Metal bindingi204 – 2041Divalent metal cation 2; catalytic By similarityUniRule annotation
Metal bindingi235 – 2351Divalent metal cation 1 By similarityUniRule annotation
Metal bindingi235 – 2351Divalent metal cation 2; catalytic By similarityUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

AminopeptidaseUniRule annotationImported, Hydrolase, Protease

Keywords - Ligandi

Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase M
Gene namesi
Name:mapUniRule annotationImported
ORF Names:APECO1_1819Imported
OrganismiEscherichia coli O1:K1 / APECImported
Taxonomic identifieri405955 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000008216: Chromosome

PTM / Processingi

Proteomic databases

PRIDEiA1A7L2.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi405955.APECO1_1819.

Structurei

3D structure databases

ProteinModelPortaliA1A7L2.
SMRiA1A7L2. Positions 2-264.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0024.
HOGENOMiHOG000030427.
KOiK01265.
OMAiEGMCFTI.
OrthoDBiEOG6MWNDS.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
HAMAPiMF_01974. MetAP_1.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
TIGRFAMsiTIGR00500. met_pdase_I. 1 hit.
PROSITEiPS00680. MAP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1A7L2-1 [UniParc]FASTAAdd to Basket

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MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV    50
NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV 100
IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG 150
AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT 200
FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL 250
RKDDTIPAII SHDE 264
Length:264
Mass (Da):29,331
Last modified:January 23, 2007 - v1
Checksum:iF2D0B57715A67851
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000468 Genomic DNA. Translation: ABI99651.1.
RefSeqiYP_851366.1. NC_008563.1.

Genome annotation databases

EnsemblBacteriaiABI99651; ABI99651; APECO1_1819.
GeneIDi4493689.
KEGGiecv:APECO1_1819.
PATRICi18211731. VBIEscCol127180_0179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000468 Genomic DNA. Translation: ABI99651.1 .
RefSeqi YP_851366.1. NC_008563.1.

3D structure databases

ProteinModelPortali A1A7L2.
SMRi A1A7L2. Positions 2-264.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405955.APECO1_1819.

Chemistry

BindingDBi A1A7L2.

Proteomic databases

PRIDEi A1A7L2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI99651 ; ABI99651 ; APECO1_1819 .
GeneIDi 4493689.
KEGGi ecv:APECO1_1819.
PATRICi 18211731. VBIEscCol127180_0179.

Phylogenomic databases

eggNOGi COG0024.
HOGENOMi HOG000030427.
KOi K01265.
OMAi EGMCFTI.
OrthoDBi EOG6MWNDS.

Family and domain databases

Gene3Di 3.90.230.10. 1 hit.
HAMAPi MF_01974. MetAP_1.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002467. Pept_M24A_MAP1.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 1 hit.
TIGRFAMsi TIGR00500. met_pdase_I. 1 hit.
PROSITEi PS00680. MAP_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes."
    Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.
    J. Bacteriol. 189:3228-3236(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APEC O1Imported.

Entry informationi

Entry nameiA1A7L2_ECOK1
AccessioniPrimary (citable) accession number: A1A7L2
Entry historyi
Integrated into UniProtKB/TrEMBL: January 23, 2007
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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